Fig. 1
From: Fluorescent proteins reveal what trypanosomes get up to inside the tsetse fly
Design of experimental crosses. Sequential experimental designs (a-c) used to investigate mating in Trypanosoma brucei. Diploid parental trypanosomes were genetically engineered to contain the genes indicated: GFP, gene for green fluorescent protein; GFPTET, GFP gene under control of the TET repressor; TET, gene for bacterial TET repressor; GFPT7, GFP gene driven by phage T7 promotor; T7 POL, gene for phage T7 polymerase; RFP, gene for modified red fluorescent protein. Parental trypanosome clones were co-transmitted through tsetse flies and the expected genotypes of hybrid progeny, assuming Mendelian inheritance, are as indicated