Fig. 3
From: A transgenic tool to assess Anopheles mating competitiveness in the field
Plugin-tdTomato localization in the MAGs and mating plug. In all panels, Plugin-tdTomato fluorescence is shown in red, Plugin in green, and DNA stained with DAPI in blue. a Plugin-tdTomato-filled vesicles occurred throughout the secretory cells of the anterior MAG compartment (ant) but was not detected in the posterior compartment (post) or aedeagus (aed). Stacked Z-projection of the top 3.5 μm. The box in the merge panel represents the region shown in (b). b Close-up of Plugin and Plugin-tdTomato where MAG epithelial integrity has been disrupted manually. Some Plugin-tdTomato vesicles are co-stained with Plugin (arrowheads). Stacked Z-projection of the top 3.5 μm. c The female reproductive tract, consisting of the ovaries (Ov), atrium (At), and the sperm storage organ spermatheca (Sp) immediately after mating. The red fluorescent mating plug is visible within the atrium. d A single Z-slice of the mating plug within the female atrium immediately post-mating. Plugin-tdTomato is visible throughout the mating plug, except the tip. Scale-bars: (a, c), 100 μm; (b), 20 μm; (d), 50 μm