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Table 3 Top 50 most abundant proteins from the soluble somatic proteome profile of adult C. daubneyi. Putative proteins were identified by 2D SDS-PAGE and LC MS/MS. The most significant match is shown for each protein spot scoring above the MASCOT score significance threshold of 47 (spot numbers designated with an * indicate the best match was below the threshold). Putative identifications in bold correspond to gene components identified in the top 50 from the transcript analysis

From: Polyomic tools for an emerging livestock parasite, the rumen fluke Calicophoron daubneyi; identifying shifts in rumen functionality

Spot Gene ID Isoform MASCOT score Uniref90 BLASTp annotation Alternative annotation UniProt description Organism Protein family
1 TR14423|c0_g1 i1 226 T1K7W8_TETUR Uncharacterized protein Tetranychus urticae (two-spotted spider mite) 0
2 TR17138|c0_g1 i1 244 A0A074ZQZ2_9TREM Uncharacterized protein Opisthorchis viverrini Calycin superfamily; fatty-acid binding protein (FABP) family
3 TR18162|c0_g1 i1 391 A0A075AK05_9TREM Uncharacterized protein Opisthorchis viverrini Calycin superfamily; fatty-acid binding protein (FABP) family
4 TR18162|c0_g1 i1 288 A0A075AK05_9TREM Uncharacterized protein Opisthorchis viverrini Calycin superfamily; fatty-acid binding protein (FABP) family
5 TR17112|c0_g1 i1 193 Q25595_CLOSI Putative glutathione transferase Clonorchis sinensis (Chinese liver fluke) GST superfamily
6 TR18162|c0_g1 i1 158 A0A075AK05_9TREM Uncharacterized protein Opisthorchis viverrini Calycin superfamily; fatty-acid binding protein (FABP) family
7 TR17170|c0_g1 i1 193 0 No annotations na na na
8 TR18162|c0_g1 i1 247 A0A075AK05_9TREM Uncharacterized protein Opisthorchis viverrini Calycin superfamily; fatty-acid binding protein (FABP) family
9 TR21279|c0_g5 i1 150 C1LY00_SCHJA Glutathione S-transferase (EC 2.5.1.18) Schistosoma japonicum (blood fluke) 0
10 TR18466|c1_g2 i1 300 Q5D2M7_9TREM Myoglobin 1 Paragonimus westermani Globin family
11 TR18162|c0_g1 i1 220 A0A075AK05_9TREM Uncharacterized protein Opisthorchis viverrini Calycin superfamily; fatty-acid binding protein (FABP) family
12 TR21279|c0_g5 i1 161 C1LY00_SCHJA Glutathione S-transferase (EC 2.5.1.18) Schistosoma japonicum (blood fluke) 0
13 TR17688|c0_g1 i2 140 T1K7W8_TETUR Uncharacterized protein Tetranychus urticae (two-spotted spider mite) 0
14 TR21279|c0_g3 i8 192 Q06A71_FASHE Glutathione transferase sigma class (EC 2.5.1.18) Fasciola hepatica (liver fluke) GST superfamily
15 TR21279|c0_g5 i1 181 C1LY00_SCHJA Glutathione S-transferase (EC 2.5.1.18) Schistosoma japonicum (blood fluke) 0
16 TR18466|c1_g2 i1 159 Q5D2M7_9TREM Myoglobin 1 Paragonimus westermani Globin family
17 TR17138|c0_g1 i1 121 A0A074ZQZ2_9TREM Uncharacterized protein Opisthorchis viverrini Calycin superfamily; fatty-acid binding protein (FABP) family
18 TR17112|c0_g1 i1 123 Q25595_CLOSI Putative glutathione transferase Clonorchis sinensis (Chinese liver fluke) GST superfamily
19 TR18162|c0_g1 i1 327 A0A075AK05_9TREM Uncharacterized protein Opisthorchis viverrini Calycin superfamily; fatty-acid binding protein (FABP) family
20 TR17982|c0_g1 i1 579 I2K1A3_DEKBR Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) Brettanomyces bruxellensis AWRI1499 Cyclophilin-type PPIase family
21 TR18162|c0_g1 i1 374 A0A075AK05_9TREM Uncharacterized protein Opisthorchis viverrini Calycin superfamily; fatty-acid binding protein (FABP) family
22 TR18466|c1_g2 i1 179 Q5D2M7_9TREM Myoglobin 1 Paragonimus westermani Globin family
23 TR16134|c0_g1 i2 174 G9P9T2_HYPAI Uncharacterized protein Hypocrea atroviridis (Trichoderma atroviride) 0
24 TR17219|c0_g1 i1 302 G4V7Z7_SCHMA Malate dehydrogenase (EC 1.1.1.37) Schistosoma mansoni (blood fluke) LDH/MDH superfamily, MDH type 2 family
25 TR17170|c0_g1 i1 153 0 No annotations na na na
26 TR17688|c0_g1 i2 176 T1K7W8_TETUR Uncharacterized protein Tetranychus urticae (two-spotted spider mite) 0
27 TR17138|c0_g1 i1 69 A0A074ZQZ2_9TREM Uncharacterized protein Opisthorchis viverrini Calycin superfamily; fatty-acid binding protein (FABP) family
28 TR17982|c0_g1 i1 426 I2K1A3_DEKBR Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) Brettanomyces bruxellensis AWRI1499 Cyclophilin-type PPIase family
29 TR18162|c0_g1 i1 317 A0A075AK05_9TREM Uncharacterized protein Opisthorchis viverrini Calycin superfamily; fatty-acid binding protein (FABP) family
30 TR17367|c0_g1 i1 924 G7YF68_CLOSI Enolase Clonorchis sinensis (Chinese liver fluke) 0
31 TR17138|c0_g1 i1 69 A0A074ZQZ2_9TREM Uncharacterized protein Opisthorchis viverrini Calycin superfamily; fatty-acid binding protein (FABP) family
32 TR21565|c1_g1 i1 386 G4LWI3_SCHMA Aldehyde dehydrogenase, putative (EC 1.2.1.5) Schistosoma mansoni (blood fluke) Aldehyde dehydrogenase family
33 TR15091|c0_g1 i1 167 Q4VVC2_CLOSI Malate dehydrogenase (EC 1.1.1.37) Clonorchis sinensis (Chinese liver fluke) LDH/MDH superfamily; MDH type 1 family
34 TR18374|c0_g1 i1 92 S4UI50_FASHE Triosephosphate isomerase (EC 5.3.1.1) Fasciola hepatica (liver fluke) Triosephosphate isomerase family
35 TR21279|c0_g3 i8 193 Q06A71_FASHE Glutathione transferase sigma class (EC 2.5.1.18) Fasciola hepatica (liver fluke) GST superfamily
36 TR19268|c0_g1 i1 266 G7YAJ9_CLOSI Tegument antigen Clonorchis sinensis (Chinese liver fluke) 0
37 TR13906|c0_g1 i1 126 0 G4VAB2_SCHMA Putative uncharacterized protein Schistosoma mansoni (blood fluke) 0
38 TR15690|c0_g1 i2 68 C8CB63_FENCH Fatty acids binding protein Fenneropenaeus chinensis (fleshy prawn) 0
39 TR15690|c0_g1 i1 249 W5LYZ8_LEPOC Uncharacterized protein Lepisosteus oculatus (spotted gar) 0
40 TR19268|c0_g1 i1 186 G7YAJ9_CLOSI Tegument antigen Clonorchis sinensis (Chinese liver fluke) 0
41 TR19739|c0_g1 i1 312 G4V6X1_SCHMA Sh3 domain grb2-like protein B1 (Endophilin B1) Schistosoma mansoni (blood fluke) 0
42 TR21565|c1_g1 i1 211 G4LWI3_SCHMA Aldehyde dehydrogenase, putative (EC 1.2.1.5) Schistosoma mansoni (blood fluke) Aldehyde dehydrogenase family
43 TR21722|c2_g1 i8 231 G7YFI1_CLOSI 3-oxoacyl-[acyl-carrier-protein] reductase Clonorchis sinensis (Chinese liver fluke) Short-chain dehydrogenases/reductases (SDR) family
44 TR17741|c0_g1 i1 725 B1NI98_FASHE Heat-shock protein 70 Fasciola hepatica (liver fluke) Heat-shock protein 70 family
45 TR19268|c0_g1 i1 95 G7YAJ9_CLOSI Tegument antigen Clonorchis sinensis (Chinese liver fluke) 0
46 TR18162|c0_g1 i1 204 A0A075AK05_9TREM Uncharacterized protein Opisthorchis viverrini Calycin superfamily; fatty-acid binding protein (FABP) family
47 TR21565|c1_g1 i1 605 G4LWI3_SCHMA Aldehyde dehydrogenase, putative (EC 1.2.1.5) Schistosoma mansoni (Blood fluke) Aldehyde dehydrogenase family
48 TR19308|c0_g1 i1 1131 G7YUU9_CLOSI Chaperonin GroEL Clonorchis sinensis (Chinese liver fluke) Chaperonin (HSP60) family
49 TR19308|c0_g1 i1 513 G7YUU9_CLOSI Chaperonin GroEL Clonorchis sinensis (Chinese liver fluke) Chaperonin (HSP60) family
50 No significant hits      na na 0
  1. Abbreviation: na not applicable