Table 3 Top 50 most abundant proteins from the soluble somatic proteome profile of adult C. daubneyi. Putative proteins were identified by 2D SDS-PAGE and LC MS/MS. The most significant match is shown for each protein spot scoring above the MASCOT score significance threshold of 47 (spot numbers designated with an * indicate the best match was below the threshold). Putative identifications in bold correspond to gene components identified in the top 50 from the transcript analysis
Spot | Gene ID | Isoform | MASCOT score | Uniref90 BLASTp annotation | Alternative annotation | UniProt description | Organism | Protein family |
|---|---|---|---|---|---|---|---|---|
1 | TR14423|c0_g1 | i1 | 226 | T1K7W8_TETUR | – | Uncharacterized protein | Tetranychus urticae (two-spotted spider mite) | 0 |
2 | TR17138|c0_g1 | i1 | 244 | A0A074ZQZ2_9TREM | – | Uncharacterized protein | Opisthorchis viverrini | Calycin superfamily; fatty-acid binding protein (FABP) family |
3 | TR18162|c0_g1 | i1 | 391 | A0A075AK05_9TREM | – | Uncharacterized protein | Opisthorchis viverrini | Calycin superfamily; fatty-acid binding protein (FABP) family |
4 | TR18162|c0_g1 | i1 | 288 | A0A075AK05_9TREM | – | Uncharacterized protein | Opisthorchis viverrini | Calycin superfamily; fatty-acid binding protein (FABP) family |
5 | TR17112|c0_g1 | i1 | 193 | Q25595_CLOSI | – | Putative glutathione transferase | Clonorchis sinensis (Chinese liver fluke) | GST superfamily |
6 | TR18162|c0_g1 | i1 | 158 | A0A075AK05_9TREM | – | Uncharacterized protein | Opisthorchis viverrini | Calycin superfamily; fatty-acid binding protein (FABP) family |
7 | TR17170|c0_g1 | i1 | 193 | 0 | No annotations | na | na | na |
8 | TR18162|c0_g1 | i1 | 247 | A0A075AK05_9TREM | – | Uncharacterized protein | Opisthorchis viverrini | Calycin superfamily; fatty-acid binding protein (FABP) family |
9 | TR21279|c0_g5 | i1 | 150 | C1LY00_SCHJA | – | Glutathione S-transferase (EC 2.5.1.18) | Schistosoma japonicum (blood fluke) | 0 |
10 | TR18466|c1_g2 | i1 | 300 | Q5D2M7_9TREM | – | Myoglobin 1 | Paragonimus westermani | Globin family |
11 | TR18162|c0_g1 | i1 | 220 | A0A075AK05_9TREM | – | Uncharacterized protein | Opisthorchis viverrini | Calycin superfamily; fatty-acid binding protein (FABP) family |
12 | TR21279|c0_g5 | i1 | 161 | C1LY00_SCHJA | – | Glutathione S-transferase (EC 2.5.1.18) | Schistosoma japonicum (blood fluke) | 0 |
13 | TR17688|c0_g1 | i2 | 140 | T1K7W8_TETUR | – | Uncharacterized protein | Tetranychus urticae (two-spotted spider mite) | 0 |
14 | TR21279|c0_g3 | i8 | 192 | Q06A71_FASHE | – | Glutathione transferase sigma class (EC 2.5.1.18) | Fasciola hepatica (liver fluke) | GST superfamily |
15 | TR21279|c0_g5 | i1 | 181 | C1LY00_SCHJA | – | Glutathione S-transferase (EC 2.5.1.18) | Schistosoma japonicum (blood fluke) | 0 |
16 | TR18466|c1_g2 | i1 | 159 | Q5D2M7_9TREM | – | Myoglobin 1 | Paragonimus westermani | Globin family |
17 | TR17138|c0_g1 | i1 | 121 | A0A074ZQZ2_9TREM | – | Uncharacterized protein | Opisthorchis viverrini | Calycin superfamily; fatty-acid binding protein (FABP) family |
18 | TR17112|c0_g1 | i1 | 123 | Q25595_CLOSI | – | Putative glutathione transferase | Clonorchis sinensis (Chinese liver fluke) | GST superfamily |
19 | TR18162|c0_g1 | i1 | 327 | A0A075AK05_9TREM | – | Uncharacterized protein | Opisthorchis viverrini | Calycin superfamily; fatty-acid binding protein (FABP) family |
20 | TR17982|c0_g1 | i1 | 579 | I2K1A3_DEKBR | – | Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) | Brettanomyces bruxellensis AWRI1499 | Cyclophilin-type PPIase family |
21 | TR18162|c0_g1 | i1 | 374 | A0A075AK05_9TREM | – | Uncharacterized protein | Opisthorchis viverrini | Calycin superfamily; fatty-acid binding protein (FABP) family |
22 | TR18466|c1_g2 | i1 | 179 | Q5D2M7_9TREM | – | Myoglobin 1 | Paragonimus westermani | Globin family |
23 | TR16134|c0_g1 | i2 | 174 | G9P9T2_HYPAI | – | Uncharacterized protein | Hypocrea atroviridis (Trichoderma atroviride) | 0 |
24 | TR17219|c0_g1 | i1 | 302 | G4V7Z7_SCHMA | – | Malate dehydrogenase (EC 1.1.1.37) | Schistosoma mansoni (blood fluke) | LDH/MDH superfamily, MDH type 2 family |
25 | TR17170|c0_g1 | i1 | 153 | 0 | No annotations | na | na | na |
26 | TR17688|c0_g1 | i2 | 176 | T1K7W8_TETUR | – | Uncharacterized protein | Tetranychus urticae (two-spotted spider mite) | 0 |
27 | TR17138|c0_g1 | i1 | 69 | A0A074ZQZ2_9TREM | – | Uncharacterized protein | Opisthorchis viverrini | Calycin superfamily; fatty-acid binding protein (FABP) family |
28 | TR17982|c0_g1 | i1 | 426 | I2K1A3_DEKBR | – | Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) | Brettanomyces bruxellensis AWRI1499 | Cyclophilin-type PPIase family |
29 | TR18162|c0_g1 | i1 | 317 | A0A075AK05_9TREM | – | Uncharacterized protein | Opisthorchis viverrini | Calycin superfamily; fatty-acid binding protein (FABP) family |
30 | TR17367|c0_g1 | i1 | 924 | G7YF68_CLOSI | – | Enolase | Clonorchis sinensis (Chinese liver fluke) | 0 |
31 | TR17138|c0_g1 | i1 | 69 | A0A074ZQZ2_9TREM | – | Uncharacterized protein | Opisthorchis viverrini | Calycin superfamily; fatty-acid binding protein (FABP) family |
32 | TR21565|c1_g1 | i1 | 386 | G4LWI3_SCHMA | – | Aldehyde dehydrogenase, putative (EC 1.2.1.5) | Schistosoma mansoni (blood fluke) | Aldehyde dehydrogenase family |
33 | TR15091|c0_g1 | i1 | 167 | Q4VVC2_CLOSI | – | Malate dehydrogenase (EC 1.1.1.37) | Clonorchis sinensis (Chinese liver fluke) | LDH/MDH superfamily; MDH type 1 family |
34 | TR18374|c0_g1 | i1 | 92 | S4UI50_FASHE | – | Triosephosphate isomerase (EC 5.3.1.1) | Fasciola hepatica (liver fluke) | Triosephosphate isomerase family |
35 | TR21279|c0_g3 | i8 | 193 | Q06A71_FASHE | – | Glutathione transferase sigma class (EC 2.5.1.18) | Fasciola hepatica (liver fluke) | GST superfamily |
36 | TR19268|c0_g1 | i1 | 266 | G7YAJ9_CLOSI | – | Tegument antigen | Clonorchis sinensis (Chinese liver fluke) | 0 |
37 | TR13906|c0_g1 | i1 | 126 | 0 | G4VAB2_SCHMA | Putative uncharacterized protein | Schistosoma mansoni (blood fluke) | 0 |
38 | TR15690|c0_g1 | i2 | 68 | C8CB63_FENCH | – | Fatty acids binding protein | Fenneropenaeus chinensis (fleshy prawn) | 0 |
39 | TR15690|c0_g1 | i1 | 249 | W5LYZ8_LEPOC | – | Uncharacterized protein | Lepisosteus oculatus (spotted gar) | 0 |
40 | TR19268|c0_g1 | i1 | 186 | G7YAJ9_CLOSI | – | Tegument antigen | Clonorchis sinensis (Chinese liver fluke) | 0 |
41 | TR19739|c0_g1 | i1 | 312 | G4V6X1_SCHMA | – | Sh3 domain grb2-like protein B1 (Endophilin B1) | Schistosoma mansoni (blood fluke) | 0 |
42 | TR21565|c1_g1 | i1 | 211 | G4LWI3_SCHMA | – | Aldehyde dehydrogenase, putative (EC 1.2.1.5) | Schistosoma mansoni (blood fluke) | Aldehyde dehydrogenase family |
43 | TR21722|c2_g1 | i8 | 231 | G7YFI1_CLOSI | – | 3-oxoacyl-[acyl-carrier-protein] reductase | Clonorchis sinensis (Chinese liver fluke) | Short-chain dehydrogenases/reductases (SDR) family |
44 | TR17741|c0_g1 | i1 | 725 | B1NI98_FASHE | – | Heat-shock protein 70 | Fasciola hepatica (liver fluke) | Heat-shock protein 70 family |
45 | TR19268|c0_g1 | i1 | 95 | G7YAJ9_CLOSI | – | Tegument antigen | Clonorchis sinensis (Chinese liver fluke) | 0 |
46 | TR18162|c0_g1 | i1 | 204 | A0A075AK05_9TREM | – | Uncharacterized protein | Opisthorchis viverrini | Calycin superfamily; fatty-acid binding protein (FABP) family |
47 | TR21565|c1_g1 | i1 | 605 | G4LWI3_SCHMA | – | Aldehyde dehydrogenase, putative (EC 1.2.1.5) | Schistosoma mansoni (Blood fluke) | Aldehyde dehydrogenase family |
48 | TR19308|c0_g1 | i1 | 1131 | G7YUU9_CLOSI | – | Chaperonin GroEL | Clonorchis sinensis (Chinese liver fluke) | Chaperonin (HSP60) family |
49 | TR19308|c0_g1 | i1 | 513 | G7YUU9_CLOSI | – | Chaperonin GroEL | Clonorchis sinensis (Chinese liver fluke) | Chaperonin (HSP60) family |
50 | No significant hits | Â | Â | Â | Â | na | na | 0 |