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Fig. 2 | Parasites & Vectors

Fig. 2

From: Wolbachia introduction into Lutzomyia longipalpis (Diptera: Psychodidae) cell lines and its effects on immune-related gene expression and interaction with Leishmania infantum

Fig. 2

Wolbachia infection (wMel strain) into mosquito and sand fly cells. Wolbachia introduction into mosquito RML-12 cells showed by absolute quantification (a) and by FISH at the 4th passage (b) and at 7th passage (c) using 40× magnification. In contrast, the decrease of infection into Lulo cells is represented by qPCR (d) and by FISH at the 4th passage (e), and at the 7th (f) in 20× objectives. In a and d, C+ represents the positive control and C- the negative control. The LL-5 cells showed similar results to Lulo cells (data not shown). The arrows show Wolbachia-stained in red and the DNA is stained in blue using DAPI

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