Fig. 4From: Identification and molecular characterization of a novel Babesia orientalis thrombospondin-related anonymous protein (BoTRAP1)Western blot analysis of BoTRAP1. a Determination of antibody response of rBoTRAP1 in water buffalo antiserum. Lane M: molecular weight marker; Lane 1: rBoTRAP1 reacted with B. orientalis positive serum; Lane 2: rBoTRAP1 probed with B. orentalis non-infected serum from water buffalo. b Determination of native BoTRAP1 in the merozoite stage. B. orientalis lysates (Lane 1) and non-infected bovine RBCs lysates (Lane 2) were probed with anti-BoTRAP1 rabbit serumBack to article page