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Table 1 Oligonucleotide primers used for the amplification of the full-length and partial BoTRAP1 genes

From: Identification and molecular characterization of a novel Babesia orientalis thrombospondin-related anonymous protein (BoTRAP1)

Primer

Sequence (5'-3')

Restriction enzyme

TRAP1-F (full)

ATGATTGGTTACAACAAAATTTGGGGCTACG

 

TRAP1-R (full)

TTAGGCTGCTTCACCCCAAATGTTATTGTC

 

TRAP1-F (partial)

TCACTAGTGAAAACCTGTATTTTCAGGGC (TEV protease site) CTCGACTTCTCCATCGTGG

SpeI

TRAP1-R (partial)

CGCTCGAGTTAGTGGCATTTTTTAATACACCCCTCTGAC

XhoI

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Parasites & Vectors

ISSN: 1756-3305

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