Fig. 4

Binding ability to human C1q of synthesized peptides derived from different size of TsPmy231-280aa. a The amino acid sequence initiating and ending number of synthesized peptides. b Peptide P1 to P9 (5 μg) were spotted onto a nitrocellulose membrane and incubated with human C1q, then probed with anti-C1q antibody. The same amount of BSA and C1q were used as controls. The binding of P2 to human C1q was further confirmed by ELISA with plates coated with different concentrations of C1q (c) or BSA (d) (0, 0.625, 1.25, 2.5, 5 and 10 μg/ml), then incubated with same amount of peptide B-P2 (10 μg/ml) and detected by HRP-conjugated streptavidin (1:10,000). Results were repeated three times, ELISA data are shown as mean ± SEM