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Table 1 Study 1. Results of examination of organs of mice inoculated with ~30 A. abstrusus third-stage larvae each at intervals after inoculation (two mice per time point) by artificial digestion and for detection of A. abstrusus DNA

From: Mice as paratenic hosts of Aelurostrongylus abstrusus

Mouse Days post-inoculation of ~30 A. abstrusus third-stage larvae
10 20 30 45 60
AD PCR AD PCR AD PCR AD PCR AD PCR
1 2 1 2 1 2 1 2 1 2
Brain 0 - 1 - 1 - 0 - 0 +
Diaphragm 0 - 0 - 0 - 0 - 0 -
Heart 0 + 0 - 0 - 0 - 0 -
Kidneys 0 - 0 - 0 - 0 - 0 -
Liver 1 - 0 - 0 - 0 - 0 -
Lungs 0 - 0 - 0 - 0 - 0 -
Spleen 1 + 0 - 0 - 0 - 1 -
Skeletal muscle 0 - 0 - 0 - 0 - 0 -
  1. Abbreviations: AD artificial digestion: actual number of A. abstrusus third-stage larvae recovered from half of an organ’s digest, PCR detection of A. abstrusus DNA by PCR; +, PCR-positive, -, PCR-negative