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Table 1 Study 1. Results of examination of organs of mice inoculated with ~30 A. abstrusus third-stage larvae each at intervals after inoculation (two mice per time point) by artificial digestion and for detection of A. abstrusus DNA

From: Mice as paratenic hosts of Aelurostrongylus abstrusus

Mouse

Days post-inoculation of ~30 A. abstrusus third-stage larvae

10

20

30

45

60

AD

PCR

AD

PCR

AD

PCR

AD

PCR

AD

PCR

1

2

1

2

1

2

1

2

1

2

Brain

0

-

1

-

1

-

0

-

0

+

Diaphragm

0

-

0

-

0

-

0

-

0

-

Heart

0

+

0

-

0

-

0

-

0

-

Kidneys

0

-

0

-

0

-

0

-

0

-

Liver

1

-

0

-

0

-

0

-

0

-

Lungs

0

-

0

-

0

-

0

-

0

-

Spleen

1

+

0

-

0

-

0

-

1

-

Skeletal muscle

0

-

0

-

0

-

0

-

0

-

  1. Abbreviations: AD artificial digestion: actual number of A. abstrusus third-stage larvae recovered from half of an organ’s digest, PCR detection of A. abstrusus DNA by PCR; +, PCR-positive, -, PCR-negative