Plasmodium vivax CSP-Pvs25 variants from southern Mexico produce distinct patterns of infectivity for Anopheles albimanus versus An. pseudopunctipennis, in each case independent of geographical origin

Table 2 The proportion of An. albimanus and An. pseudopunctipennis mosquitoes (from various geographical sites) infected with oocysts and the corresponding oocyst density produced by different P. vivax genotypes (cspVk /Pvs25-130) from southern Mexico

Geographical site and mosquito colony	Vk210/Pvs25-A			Vk210/Pvs25-B			Vk247/Pvs25-B
Geographical site and mosquito colony	n	Prop. infected	Median number of oocysts (IQR)	n	Prop. infected	Median number of oocysts (IQR)	n	Prop. infected	Median number of oocysts (IQR)
An. albimanus
1. A/WS-R	210	0.72	14 (6.0–28.0)	280	0.10	7 (4.0–17.0)	1133	0.17	4 (1.0–15.0)
1. Coast, Chiapas	–	–	–	65	0.0 ^0.001	15.0 (15.0–15.0)	70	0.11 ^0.285	4 (2.5–5.0)
2. LF, Chiapas	10	0.40 ^0.067	5 (2.5–10.0)	45	0.07 ^0.519	2.0 (2.0–17.0)	205	0.03 ^<0.001	9 (3.0–10.0)
3. Oaxaca	10	0.80 ^0.690	18.5 (7.0–25.0)	56	0.04 ^0.260	7.0 (6.0–8.0)	119	0.01 ^0.001	66 (66.0–66.0)
4. Veracruz	10	0.40 ^0.067	11 (4.0–23.5)	20	0.0	–	35	0.06 ^0.233	3.5 (1.0–6.0)
An. pseudopunctipennis
1. P/TAP-R	113	0.03	2 (1.0–3.0)	147	0.60	17 (7.0–39.0)	415	0.80	41 (17.0–72.0)
3. Oaxaca	28	0.07 ^0.398	12.5 (9.0–16.0)	87	0.55 ^0.552	16.5 (7.0–39.5)	80	0.79 ^0.765	33 (21.0–57.0)
5. Nuevo Leon	75	0.03 ^0.997	1 (1.0–1.0)	59	0.53 ^0.391	19 (8.0–41.0)	275	0.81 ^0.876	39 (18.0–63.0)
6. Guatemala	68	0.09 ^0.135	1.5 (1.0–3.0)	100	0.56 ^0.622	20.5 (8.5–36.5)	207	0.71 ^0.015	27 (10.5–53.0)^a

Notes: Only infected mosquitoes were considered for the calculation of the median number of oocysts and its IQR. P-values from the comparison of the proportion of infected mosquitoes of a colony against the reference strain are indicated as superscripts (underlined are those having significance)
^aCompared to the reference mosquitoes, the number of oocysts was significantly lower in the Guatemala colony (P = 0.002) when fed with parasite genotype Vk247/Pvs25-B. Statistical significance was calculated by using the Jackknife coefficient variance estimator at 95% confidence interval (according to models described in method section)
Abbreviations: n total number of mosquitoes examined, Prop. proportion, A/WS-R reference strain of An. albimanus, P/TAP-R reference strain of An. pseudopunctipennis, LF Lacandon Forest, IQR interquartile range

ISSN: 1756-3305