Fig. 3
From: Molecular characterisation and expression analysis of two heat-shock proteins in Taenia multiceps
SDS-PAGE and western blot analysis of Tm-HSP60 (a) and Tm-p36 (b). Lane M: protein molecular weight markers; Lane A: crude extracts of Escherichia coli expressing pET32a(+) induced by IPTG; Lane B: crude extracts of E. coli expressing pET32a(+)-Tm-HSP60/Tm-p36 induced by IPTG; Lane C: purified recombinant proteins (10 µg); Lane D: western blot of recombinant proteins (10 µg) incubated with polyclonal antibodies; Lane E: western blot of total T. multiceps adult worm extract (30 µg) probed with polyclonal antibodies (arrow indicates the location of specific band); Lane F: western blot of purified recombinant proteins (10 µg) incubated with coenurus cerebralis-infected goat serum (arrow indicates the location of specific band); Lane G: negative control goat serum