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Table 2 A list of identified female salivary gland proteins of Ae. aegypti PMD, PMD-R, and UPK-R strains by NanoLC-MS analysis

From: Protein expression in female salivary glands of pyrethroid-susceptible and resistant strains of Aedes aegypti mosquitoes

SNa (MW/pI)

Strain

Accession numberb

Protein description

Prot. scorec

PSd

No. P/ %Ce

Database MW/pI

Peptide sequence(s)

Gene namef

1 (75/5.3)

PMD

XP_011493320

AAEL017349-PA

65

36

2/3

72.4/5.1

R.LTPEDIER.M

AAEL017349-PA

29

K.VADDDKATMEEAIDEK.I + Oxidation (M)

PMD-R

XP_011493320

AAEL017349-PA

65

36

2/3

72.4/5.1

R.LTPEDIER.M

AAEL017349-PA

29

K.VADDDKATMEEAIDEK.I + Oxidation (M)

UPK-R

XP_011493320

AAEL017349-PA

271

66

4/7

72.4/5.1

K.IVITNDQNR.L

AAEL017349-PA

71

K.FEELNMDLFR.S + Oxidation (M)

63

K.DVDEIVLVGGSTR.I

75

K.VFAPEEISAMVLGK.M + Oxidation (M)

2 (73/5.6)

PMD

ABF18332

Heat-shock cognate 70

40

40

1/1

71.4/5.3

K.VEIIANDQGNR.T

nag

PMD-R

ABF18332

Heat-shock cognate 70

49

49

1/1

71.4/5.3

K.VEIIANDQGNR.T

na

UPK-R

ABF18332

Heat-shock cognate 70

163

80

2/3

71.4/5.3

K.DAGTISGLNVLR.I

na

83

R.FEELNADLFR.S

3 (72/6.2)

PMD

AAL76020

Putative secreted protein

84

84

1/1

66.6/5.8

K.TASNDCTPFR.L

AAEL000748

PMD-R

AAL76020

Putative secreted protein

84

84

1/1

66.6/5.8

K.TASNDCTPFR.L

AAEL000748

UPK-R

AAL76020

Putative secreted protein

130

62

2/4

66.6/5.8

K.DLEGITDELGK.V

AAEL000748

67

K.DIATLEQNVNTLHK.D

4 (69/8.5)

PMD

AAC37218

Apyrase

231

65

3/6

63.2/8.5

R.TGPLDSDVFK.N

APY

82

K.VTLSNAVEAVR.R

84

K.VEAIGSTVVGETK.I

PMD-R

AAC37218

Apyrase

449

66

6/12

63.2/8.5

R.TGPLDSDVFK.N

APY

61

R.TNCLQVSGLR.I

76

K.VTLSNAVEAVR.R

73

K.VEAIGSTVVGETK.I

96

K.KIEVMDYTNPK.S + Oxidation (M)

80

K.DKVEGPYPTIVESK.N

UPK-R

AAC37218

Apyrase

231

81

3/5

63.2/8.5

K.IGIIGVLYDK.T

APY

72

R.IVIDISKPIR.S

78

K.VTLSNAVEAVR.R

5 (68/8.9)

PMD

AAC37218

Apyrase

137

59

2/4

63.2/8.5

R.TNCLQVSGLR.I

APY

75

K.VEAIGSTVVGETK.I

PMD-R

AAC37218

Apyrase

531

66

7/12

63.2/8.5

R.TGPLDSDVFK.N

APY

71

K.IGIIGVLYDK.T

69

R.TNCLQVSGLR.I

87

K.VTLSNAVEAVR.R

68

R.RTGPLDSDVFK.N

79

K.VEAIGSTVVGETK.I

93

K.DKVEGPYPTIVESK.N

UPK-R

AAC37218

Apyrase

641

61

9/17

63.2/8.5

R.TGPLDSDVFK.N

APY

81

K.IGIIGVLYDK.T

64

R.TNCLQVSGLR.I

70

R.IVIDISKPIR.S

79

K.VTLSNAVEAVR.R

59

K.GLAPYLAELNK.E

60

K.VEAIGSTVVGETK.I

80

K.KIEVMDYTNPK.S + Oxidation (M)

63

K.DKVEGPYPTIVESK.N

6 (68/9.5)

PMD

XP_001651903

AAEL006333-PA

139

80

2/3

63.9/8.7

R.ISLAAGAITR.G

AAEL006333

61

K.LELSNDCR.L

PMD-R

XP_001651903

AAEL006333-PA

139

80

2/3

63.9/8.7

R.ISLAAGAITR.G

AAEL006333

61

K.LELSNDCR.L

UPK-R

ABF18486

5’-nucleotidase

96

20

3/6

61.8/8.9

R.VTVLGSTIK.K

AAEL006333

38

K.VGESKLELSNDCR.L

39

R.GQILTALPFNSNANR.V

7 (66/7.2)

PMD

XP_001651237

AAEL005672-PA

593

37

11/23

59.9/6.4

R.LKLEEMVK.G + Oxidation (M)

AAEL005672

48

R.HIFEVLDR.F

45

R.FATDDEFLK.I

35

K.SNHPEFIGAK.F

51

K.EWSLVSEIR.Q

51

R.SLLEFAPALLK.L

52

K.GDFGVSHGPQFK.C

70

R.GVLPDVYDLDGK.I

87

K.TDQNLIDAVLLGSK.R

62

K.QFYDDHVQYLEFR.G

54

K.QLALNSIEYSAMNSEEK.T + Oxidation (M)

PMD-R

AAL76033

Putative adenosine deaminase

100

22

3/4

60.6/6.5

K.FIYAPGR.F

AAEL005672

36

K.SSHPEFIGAK.F

45

R.FATDDEFLK.I

UPK-R

AAL76033

Putative adenosine deaminase

219

70

3/7

60.6/6.5

R.GVLPDVYDLDGK.I

AAEL005672

87

K.TDQNLIDAVLLGSK.R

62

K.QFYDDHVQYLEFR.G

8 (62/5.1)

PMD

XP_001649774

AAEL000641-PA

163

77

2/4

56.3/5.0

K.MDATANELEHTK.I + Oxidation (M)

AAEL000641

86

K.VDATEEQELSEK.H

PMD-R

XP_001649774

AAEL000641-PA

47

47

1/2

56.3/5.0

R.NGTPIEYTGGR.E

AAEL000641

UPK-R

XP_001649774

AAEL000641-PA

82

82

1/2

56.3/5.0

K.VDATEEQELSEK.H

AAEL000641

9 (59/6.6)

PMD

ABF18028

Salivary anti-FXa serpin

121

59

2/5

48.3/6.1

R.MGQVINDALK.N + Oxidation (M)

SRPN23

62

K.TPPEAAMGLEDK.Q + Oxidation (M)

PMD-R

ABF18028

Salivary anti-FXa serpin

58

58

1/2

48.3/6.1

R.TAMAVAIGSEK.V + Oxidation (M)

SRPN23

UPK-R

ABF18028

Salivary anti- FXa serpin

307

59

4/11

48.3/6.1

R.TAMAVAIGSEK.V + Oxidation (M)

SRPN23

77

R.MGQVINDALK.N + Oxidation (M)

106

R.IPQFGLQTTVPGR.Q

64

K.VFEQGQDVALGEIVQK.M

10 (49/5.1)

PMD

AAL76010

Putative purine hydrolase

48

48

1/2

38.2/5.0

R.QMVEDLNR.- + Oxidation (M)

AAEL006485

PMD-R

AAL76010

Putative purine hydrolase

76

42

2/4

38.2/5.0

R.LITPAPR.R

AAEL006485

34

R.QMVEDLNR.- + Oxidation (M)

UPK-R

AAL76010

Putative purine hydrolase

73

73

1/3

38.2/5.0

K.SEIAGIYILGGNR.N

AAEL006485

11 (48/6.0)

PMD

ABF18025

Angiopoietin-like protein variant, partial

68

22

2/6

33.5/5.7

K.FVVGPEEQR.Y

AAEL000749

45

K.FSDVSNTPLK.L

PMD-R

AAL76032

Angiopoietin-like protein

68

22

2/6

33.5/5.7

K.FVVGPEEQR.Y

AAEL000749

45

K.FSDVSNTPLK.L

UPK-R

ABF18025

Angiopoietin-like protein variant, partial

153

37

4/15

33.5/5.7

K.FVVGPEEQR.Y

AAEL000749

32

K.FSDVSNTPLK.L

35

K.SSGLTTIPIGSEPR.V

49

R.FTQMFSQQFYR.H + Oxidation (M)

12 (45/5.8)

PMD

ABF18018

Putative 34 kDa secreted protein

64

64

1/3

36.4/5.2

K.LQEEIEEQTK.Q

AAEL003601

PMD-R

ABF18018

Putative 34 kDa secreted protein

64

64

1/3

36.4/5.2

K.LQEEIEEQTK.Q

AAEL003601

UPK-R

ABF18018

Putative 34 kDa secreted protein

132

65

2/6

36.4/5.2

K.SVATEIVQMR.D + Oxidation (M)

AAEL003601

68

K.QNFEDQVNQIVK.S

13 (46/5.7)

PMD

AAL76018

Putative 34 kDa secreted protein

54

54

1/4

36.4/5.2

R.DSAISTDQVDQLK.Q

AAEL003601

PMD-R

AAL76018

Putative 34 kDa secreted protein

155

74

2/7

36.4/5.2

R.DSAISTDQVDQLK.Q

AAEL003601

81

K.QNFEDQVNQIVK.S

UPK-R

AAL76018

Putative 34 kDa secreted protein

176

40

4/12

36.4/5.2

R.IYLGALR.F

AAEL003601

54

K.SVATEIVQMR.D + Oxidation (M)

51

K.VTELEQQIAK.Q

35

K.QNFEDQVNQIVK.S

14 (43/6.2)

PMD

ABF18017

Putative 34 kDa family secreted salivary protein

98

49

2/6

36.0/6.1

K.LQEEIEEQTK.Q

AAEL003600

53

K.QCNLSEDDLTK.L

PMD-R

ABF18017

Putative 34 kDa family secreted salivary protein

206

73

3/12

36.0/6.1

K.QCNLSEDDLTK.L

AAEL003600

75

K.TQGVSNMEVQLLR.E + Oxidation (M)

60

R.QANQDTSKAEGEMVEK.I + Oxidation (M)

UPK-R

ABF18017

Putative 34 kDa family secreted salivary protein

256

66

3/8

36.0/6.1

K.VLNTILDQVNK.L

AAEL003600

86

R.KVLNTILDQVNK.L

106

K.TQGVSNMEVQLLR.E

15 (40/9.5)

PMD

AAL16049

Long form D7Bclu1 salivary protein

450

64

6/18

39.2/9.4

K.NELDVDEIAR.D

AAEL006417

93

R.SKDYFAALTGK.L

74

K.SSSCSEVFEAFK.K

75

R.KQVDDIDKIQCS.-

63

K.SSSCSEVFEAFKK.V

84

K.LEPSDDQATQCYTK.C

PMD-R

XP_001657778

AAEL006417-PA

614

58

8/29

39.2/9.4

K.IGFYEPGEKR.F

AAEL006417

91

R.SKDYFAALTGK.L

68

K.YQMGSGIVFER.H + Oxidation (M)

83

K.SSSCSEVFEAFK.K

72

R.KQVDDIDKIQCS.-

68

K.VTNDFKEAFDYR.E

88

K.LEPSDDQATQCYTK.C

88

R.YMTSKNELDVDEIAR.D + Oxidation (M)

UPK-R

AAL16049

Long form D7Bclu1 salivary protein

385

63

5/14

39.2/9.4

K.AALQNWLGWK.L

AAEL006417

82

R.SKDYFAALTGK.L

87

K.SSSCSEVFEAFK.K

60

K.SSSCSEVFEAFKK.V

94

K.LEPSDDQATQCYTK.C

16 (39/9.5)

PMD

AAA29347

D7 protein

437

72

6/21

37.6/8.4

K.SKVEAYANAVK.Q

D7

71

K.FDASVIQEQFK.A

58

R.QYTVLDDALFK.E

75

K.DNQLDVEEVKR.D

85

K.CLVESSVKDDFK.E

83

R.CMEDNLEDGANR.L + Oxidation (M)

PMD-R

AAA29347

D7 protein

363

66

5/17

37.6/8.4

K.SKVEAYANAVK.Q

D7

60

K.DDFKEAFDYR.E

69

K.FDASVIQEQFK.A

72

K.QSYFEFCENK.Y

102

K.QLPSTNNDCAAVFK.A

UPK-R

AAA29347

D7 protein

390

61

6/21

37.6/8.4

R.TGLYDPVAQK.F

D7

68

K.SKVEAYANAVK.Q

71

K.FDASVIQEQFK.A

67

R.QYTVLDDALFK.E

63

K.DNQLDVEEVKR.D

70

K.QLPSTNNDCAAVFK.A

17 (40/8.9)

PMD

AAA29348

D7 protein

153

55

3/9

37.5/8.5

R.TGLYDPVAQK.F

D7

75

K.NNELDAEEVKR.D

23

K.KSYFEFCENK.Y

PMD-R

AAA29348

D7 protein

191

65

3/10

37.5/8.5

K.FDASVIQEQFK.A

D7

71

R.QYTVLDDALFK.E

59

K.NNELDAEEVKR.D

UPK-R

AAA29347

D7 protein

223

67

3/10

37.6/8.4

R.QYTVLDDALFK.E

D7

84

K.DNQLDVEEVKR.D

76

K.CLVESSVKDDFK.E

18 (42/8.9)

PMD

AAA29348

D7 protein

75

75

1/3

37.5/8.5

K.NNELDAEEVKR.D

D7

PMD-R

AAA29348

D7 protein

208

63

3/9

37.5/8.5

K.DDFKEAFDYR.E

D7

73

K.FDASVIQEQFK.A

74

K.KSYFEFCENK.Y

UPK-R

AAA29348

D7 protein

211

74

3/9

37.5/8.5

K.FDASVIQEQFK.A

D7

67

R.QYTVLDDALFK.E

74

K.KSYFEFCENK.Y

19 (33/9.5)

PMD

XP_001651000

AAEL000793-PA

199

87

2/16

29.6/9.1

R.MPTLVWDDELAHIASFNAR.K + Oxidation (M)

AAEL000793

112

R.RGPHVACNAPTQFGSACGQEPK.F

PMD-R

AAL76012

Putative secreted protein

199

87

2/16

29.6/9.1

R.MPTLVWDDELAHIASFNAR.K + Oxidation (M)

AAEL000793

112

R.RGPHVACNAPTQFGSACGQEPK.F

UPK-R

AAL76009

Putative secreted protein

38

38

1/3

30.2/9.0

K.DYCSSVFR.K

AAEL003053

20 (33/4.6)

PMD

AAX54871

Putative 19.6 kDa secreted protein

31

31

1/4

21.8/4.8

K.VIEQLDQIK.V

AAEL010228

PMD-R

XP_001660690

AAEL010228-PA

166

62

2/12

21.8/4.8

K.DSNAYQCSQDR.S

AAEL010228

104

R.NPIVDVIGSAGDFSK.I

UPK-R

XP_001660690

AAEL010228-PA

173

66

2/12

21.8/4.8

K.DSNAYQCSQDR.S

AAEL010228

107

R.NPIVDVIGSAGDFSK.I

21 (31/4.1)

PMD

ABF18125

30 kDa salivary gland allergen variant 3

124

64

2/6

27.9/4.2

R.VPVVEAIGR.I

AAEL010235

61

K.NDPADTYR.Q

PMD-R

ABF18125

30 kDa salivary gland allergen variant 3

452

64

6/18

27.9/4.2

R.VPVVEAIGR.I

AAEL010235

61

R.QVVALLDKDTK.V

86

R.SALNNDLQSEVR.V

75

K.SEYQCSEDSFAAAK.S

80

K.KSEYQCSEDSFAAAK.S

88

K.KKSEYQCSEDSFAAAK.S

UPK-R

ABF18125

30 kDa salivary gland allergen variant 3

301

61

4/17

27.9/4.2

K.NDPADTYR.Q

AAEL010235

62

R.QVVALLDKDTK.V

107

R.SALNNDLQSEVR.V

71

K.SEYQCSEDSFAAAK.S

22 (15/9.5)

PMD

AAL16050

Short form D7Cclu23 salivary protein

32

32

1/6

18.2/9.0

R.FFTEKPFCK.-

AAEL006423

PMD-R

AAL16050

Short form D7Cclu23 salivary protein

163

47

4/22

18.2/9.1

K.INGWAYFR.C

AAEL006423

46

R.FFTEKPFCK.-

54

R.NFLCHTANFK.L

16

K.TEMGDKINGWAYFR.C + Oxidation (M)

UPK-R

AAL16050

Short form D7Cclu23 salivary protein

32

32

1/6

18.2/9.1

R.FFTEKPFCK.-

AAEL006423

23 (15/5.6)

PMD

AAL76029

Putative C-type lectin

33

33

1/6

18.1/5.3

K.QQQVEELAQR.V

CTL16

PMD-R

XP_001647974

AAEL000533-PA

87

46

2/12

18.1/5.3

R.LAVLDSEQK.Q

CTL16

40

K.QQQVEELAQR.V

UPK-R

AAL76029

Putative C-type lectin

93

51

2/12

18.1/5.3

R.LAVLDSEEK.Q

CTL16

42

K.QQQVEELAQR.V

24 (15/5.9)

PMD

AAL76035

Putative 16.9 kDa secreted protein

135

23

3/20

17.4/5.5

K.TELDEVTK.E

SG14.5sp

32

R.NLQVDGNMPK.V + Oxidation (M)

80

K.VAQDMVPYGFNIK.T + Oxidation (M)

PMD-R

ABF18031

Short salivary D7 protein, partial

80

80

1/8

17.5/5.7

K.VAQDMVPYGFNIK.T + Oxidation (M)

na

UPK-R

ABF18031

Short salivary D7 protein, partial

75

75

1/8

17.5/5.7

K.VAQDMVPYGFNIK.T + Oxidation (M)

na

A1 (20/8.6)

PMD

ABF18082

Short D7 protein

44

44

1/5

18.6/8.2

R.QNQIEELNK.L

SG16.2sp

A2 (56/6.2)

PMD-R

ABF18028

Salivary anti-FXa serpin

229

70

3/8

48.3/6.1

R.TAMAVAIGSEK.V + Oxidation (M)

SRPN23

69

K.MEMSIAADGEK.Q + Oxidation (M)

90

K.TPPEAAMGLEDK.Q + Oxidation (M)

UPK-R

ABF18028

Salivary anti-FXa serpin

95

95

1/3

48.3/6.1

R.IPQFGLQTTVPGR.Q

SRPN23

A4 (59/6.2)

UPK-R

ABF18509

Salivary serpin

235

62

3/8

48.2/6.3

R.MGQVINDALK.N + Oxidation (M)

SRPN23

68

K.TPPEAGMGLEDK.Q + Oxidation (M)

105

R.IPQFGLQTTVPGR.Q

  1. aSpot number refers to those shown in Fig. 2 /(Observed MW/pI)
  2. bAccession number of the best hit of proteins from Ae. aegypti, available at http://www.ncbi.nlm.nih.gov
  3. cProt. Score = Protein score, Mowse score ≥ 30
  4. dPS = Peptide score
  5. eNo. P/%C = Number of peptide/% coverage
  6. fName of the gene that codes for the protein sequences, available at http://www.uniprot.org/uploadlists
  7. gna, not available
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