Fig. 3
From: Efficient genome engineering of Toxoplasma gondii using the TALEN technique
Identification of in vitro-induced bradyzoites and expression of inserted genes in transgenic T. gondii. RT-PCR verification of the tachyzoite-specific protein SAG1 and bradyzoite-specific protein BAG1 in PRU/AAH2-eGFP tachyzoites and in vitro-induced bradyzoites, respectively. RT-PCR verification of the expression of the inserted genes eGFP and DHFR* compared with the PCR verification of the inserted genes from transgenic T. gondii gDNA