Fig. 5
From: Efficient genome engineering of Toxoplasma gondii using the TALEN technique
Identification of transgenic T. gondii cysts and expression of inserted genes in infected brain tissue. PCR verification of inserted DHFR* from different templates of PRU/AAH2-eGFP. PCR verification of AAH2-eGFP from different templates of PRU/AAH2-eGFP. RT-PCR verification of bradyzoite-specific BAG1 in PRU/AAH2-eGFP-infected brain tissue indicated the formation of cysts