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Table 1 The efficacy and repeatability of the microfluidic device in the laboratory

From: Diagnosis of feline filariasis assisted by a novel semi-automated microfluidic device in combination with high resolution melting real-time PCR

Microfilariae level/50 µl blood

Number of microfilariae

Intra-assay variation

Inter-assay variation

Trapped mf (mean ± SD)

CV (%)

Leaked mf (mean ± SD)

CV (%)

Trapped mf (mean ± SD)

CV (%)

Leaked mf (mean ± SD)

CV (%)

High (mean 30)

27.22 ± 2.94

10.8

1.25 ± 0.83

66.4

31 ± 1.22

4.44

1.61 ± 0.55

34.16

Moderate (mean 15.5)

15.44 ± 1.3

8.42

1.56 ± 1.2

76.9

15.6 ± 0.89

4.16

1.4 ± 0.55

32.49

Low (mean 7.5)

5.25 ± 0.5

9.38

0.5 ± 0.58

94.6

5.8 ± 0.45

4.66

0.4 ± 0.55

45.8

  1. Abbreviations: CV, coefficient of variation; mf, microfilariae; SD, standard deviation