Fig. 2
From: Identification and characterization of Pv50, a novel Plasmodium vivax merozoite surface protein
Recombinant Pv50 protein for expression. a The purification progress of Pv50 (46Â kDa) was evaluated by 12.5% SDS-PAGE. b Western blot analysis of recombinant Pv50 with penta anti-His antibody (His), mouse immune sera (M), mixed vivax patient sera (P), rabbit immune sera (R), and schizont-stage parasite lysates (PL) under reducing conditions probed with IgG antibody from Pv50 rabbit immune sera. Arrowheads indicate specific bands for each recombinant protein. Abbreviations: M, protein marker; T, total translation mix; S: supernatant; P, purification; Ft: flow through; N, elution under non-reducing; R, elution under reducing