Fig. 5
From: Identification and characterization of Pv50, a novel Plasmodium vivax merozoite surface protein
Immunogenicity analysis of Pv50. OD450 value of culture medium with recombinant Pv50 (2.5, 5 and 10 µg/ml), ConA, LPS, or only culture medium as control. a Proliferation of splenocytes is shown after stimulation with Con A or LPS as a positive control. The results are expressed as the mean value of OD450 ± SD. b Cytokine levels from 72 h cultured supernatants of splenocytes from Pv50-immunized BALB/c mice stimulated in vitro with Pv50. The black horizontal bars represent the cytokine geometric means ± 3SD. c Specific IgG1 (black), IgG2b (dark gray), IgG3 (light gray) and IgG2a (white) against the immunogen itself were determined by ELISA. The results are expressed as the mean titers ± SD