Downregulation of female doublesex expression by oral-mediated RNA interference reduces number and fitness of Anopheles gambiae adult females

Table 1 Sequences of PCR primers

Gene	Vector base ID	Primer name	Primer sequence 5′–3′	Efficiency (%)
Rps7	AGAP010592	S7qf1	AGAACCAGCAGACCACCATC	115.02
Rps7	AGAP010592	S7qr1	GCTGCAAACTTCGGCTATTC	115.02
Actin5C	AGAP000651	ACT-2f	TACAACTCGATCATGAAGTGCGA	110.67
Actin5C	AGAP000651	ACT-3r	CCCGGGTACATGGTGGTACCGCCGGA	110.67
Elongation Factor	AGAP005128	EFf1	GGCAAGAGGCATAACGATCAATGCG	112.60
Elongation Factor	AGAP005128	EFr1	GTCCATCTGCGACGCTCCGG	112.60
Female doublesex	AGAP004050	newDSX-f	AGAGGGCGGGGAAATTCTAGT	111.19
Female doublesex	AGAP004050	newDSX-r	GGGCTTGTGGCAGTACGAATA	111.19
Female doublesex	AGAP004050	dsRNA_dsx-f2 (dsx1586)	CAAGCGGTGGTCAACGAATA	na
Female doublesex	AGAP004050	dsRNA_dsx-r3 (dsx1589)	GCCCACTCCTTAAACACTACTT	na
Female doublesex	AGAP004050	T7dsRNA_dsx-f2	TAATACGACTCACTATAGGGCAAGCGGTGGTCAACGAATA	na
Female doublesex	AGAP004050	T7dsRNA_dsx-r3	TAATACGACTCACTATAGGGGCCCACTCCTTAAACACTACTT	na

ISSN: 1756-3305