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Table 1 Primers used for the amplification of the partial BoTRAP2 genes

From: Identification of a novel thrombospondin-related anonymous protein (BoTRAP2) from Babesia orientalis

Primer

Primer sequence (5′–3′)

Restriction enzyme

BoTRAP2-F

ATAATGGACTTCGTGAGTTTGTTGAAG

 

BoTRAP2-R

TACTGTTCGGACGATATTTATCGTATC

 

BoTRAP2-1F

GAATTCGTGAGTTTGTTGAAGAACTGACTAAG

EcoRI

BoTRAP2-1R

CTCGAGAGGACCCAGAAGCTCTACCTTCAAC

XhoI