Fig. 2

Variation of total erythrocyte, lymphocyte, IgM⁺ B cell, monocyte, neutrophil and thrombocyte numbers as a response of common carp to intraperitoneal S. molnari blood stage injection. Data based on Hayem-diluted blood (erythrocytes) and differential blood cell counts (2 × 100 leukocytes + thrombocytes) counted on Kwik–Diff-stained blood smears or by flow cytometry (IgM⁺ B cells; repeat experiment). Note the highly significant increase in lymphocytes from 28 dpi and IgM⁺ B cells from 35 dpi. Statistical analyses are based on data-specific LMM developed in R with significance levels *P < 0.05, **P < 0.01 and ***P < 0.001 relative to the control group. Box plots show mean ± standard deviation (box) with maximum and minimum ranges (lines)