Evaluation of emodepside in laboratory models of human intestinal nematode and schistosome infections

Parasites & Vectors

Table 1 Mean IC₅₀ values in vitro of emodepside tested on larval and adult stages of different helminths

Species	Replicates	No. of parasites per well^a	24 hours	48 hours	72 hours
Species	Replicates	No. of parasites per well^a	Mean IC₅₀ ± SD (µM)	Mean IC₅₀ ± SD (µM)	Mean IC₅₀ ± SD (µM)
T. muris (L1)	9	20–40	3.73 ± 6.54	–	–
T. muris (adults)	2	2–3	0.28 ± 0.15	0.043 ± 0.0089	0.022 ± 0.013
H. polygyrus (L3)	2	30	0.78 ± 0.086	0.9 ± 0.034	0.48 ± 0.05
H. polygyrus (adults)	3	3–4	0.57 ± 0.42	0.21 ± 0.13	0.25 ± 0.16
A. ceylanicum (L3)	2	30	0.14 ± 0.041	0.086 ± 0.08	0.25 ± 0.051
A. ceylanicum (adults)	3	2–3	0.0044 ± 0.0021	0.0015 ± 0.00078	0.0024 ± 0.002
N. americanus (L3)	2	30	0.77 ± 0.52	0.15 ± 0.069	0.083 ± 0.033
N. americanus (adults)	2	2–3	0.0031 ± 0.0011	0.0029 ± 0.0018	0.0021 ± 0.0012
S. ratti (L3)	4	30	0.73 ± 0.5	0.27 ± 0.21	0.25 ± 0.14
S. ratti (adults)	3	5–15	0.75 ± 0.57	0.21 ± 0.29	0.36 ± 0.32
S. mansoni (NTS)	2	100	7.79 ± 1.57	6.92 ± 0.21	2.48 ± 0.78
S. mansoni (adults)	2	2–3	50.4 ± 3.32	37.27 ± 10.47	34.1 ± 9.18
S. haematobium (adults)	2	2–3	40.51 ± 24.96	40.25 ± 6.49	36.73 ± 6.49

^aEach assay included 2 to 3 wells per concentration/condition
Notes: The inclusion criteria used in our analysis were different for each stage and parasite. Minimal survival rates (larvae) or viability scores (adults and NTS) and IC₅₀ r-values considered acceptable were as follows: T. muris L1 (survival rate: 60%; R = 0.7), adults (score: 2.5; R = 0.8); H. polygyrus L3 (70%; 0.9), adults (1.9; 0.8); A. ceylanicum L3 (55%; 0.7), adults (2; 0.7); N. americanus L3 (60%; 0.75), adults (2; 0.8); S. ratti L3 (60%; 0.75), adults (2; 0.7); S. mansoni NTS (2; 0.75), adults (1.5; 0.85); S. haematobium adults (2; 0.7)
Abbreviation: SD, standard deviation

ISSN: 1756-3305