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Table 1 Mean IC50 values in vitro of emodepside tested on larval and adult stages of different helminths

From: Evaluation of emodepside in laboratory models of human intestinal nematode and schistosome infections

Species Replicates No. of parasites per wella 24 hours 48 hours 72 hours
Mean IC50 ± SD (µM) Mean IC50 ± SD (µM) Mean IC50 ± SD (µM)
T. muris (L1) 9 20–40 3.73 ± 6.54
T. muris (adults) 2 2–3 0.28 ± 0.15 0.043 ± 0.0089 0.022 ± 0.013
H. polygyrus (L3) 2 30 0.78 ± 0.086 0.9 ± 0.034 0.48 ± 0.05
H. polygyrus (adults) 3 3–4 0.57 ± 0.42 0.21 ± 0.13 0.25 ± 0.16
A. ceylanicum (L3) 2 30 0.14 ± 0.041 0.086 ± 0.08 0.25 ± 0.051
A. ceylanicum (adults) 3 2–3 0.0044 ± 0.0021 0.0015 ± 0.00078 0.0024 ± 0.002
N. americanus (L3) 2 30 0.77 ± 0.52 0.15 ± 0.069 0.083 ± 0.033
N. americanus (adults) 2 2–3 0.0031 ± 0.0011 0.0029 ± 0.0018 0.0021 ± 0.0012
S. ratti (L3) 4 30 0.73 ± 0.5 0.27 ± 0.21 0.25 ± 0.14
S. ratti (adults) 3 5–15 0.75 ± 0.57 0.21 ± 0.29 0.36 ± 0.32
S. mansoni (NTS) 2 100 7.79 ± 1.57 6.92 ± 0.21 2.48 ± 0.78
S. mansoni (adults) 2 2–3 50.4 ± 3.32 37.27 ± 10.47 34.1 ± 9.18
S. haematobium (adults) 2 2–3 40.51 ± 24.96 40.25 ± 6.49 36.73 ± 6.49
  1. aEach assay included 2 to 3 wells per concentration/condition
  2. Notes: The inclusion criteria used in our analysis were different for each stage and parasite. Minimal survival rates (larvae) or viability scores (adults and NTS) and IC50 r-values considered acceptable were as follows: T. muris L1 (survival rate: 60%; R = 0.7), adults (score: 2.5; R = 0.8); H. polygyrus L3 (70%; 0.9), adults (1.9; 0.8); A. ceylanicum L3 (55%; 0.7), adults (2; 0.7); N. americanus L3 (60%; 0.75), adults (2; 0.8); S. ratti L3 (60%; 0.75), adults (2; 0.7); S. mansoni NTS (2; 0.75), adults (1.5; 0.85); S. haematobium adults (2; 0.7)
  3. Abbreviation: SD, standard deviation