Table 1 Primers and morpholino used in this study
From: Role of gamma-giardin in ventral disc formation of Giardia lamblia
Name (GiardiaDB ID) | Nucleotide sequence (5′–3′)a,b |
|---|---|
Transgenic G. lamblia expressing HA-tagged Glγ-giardin | |
γ-giardin-NcoI-F (GL50803_17230) | CATCCCATGGATGAAGTCATCGTTCTCGACCG |
γ-giardin-HAX3-R (GL50308_17230) | GTTACGCGGCCGCTTAAGCGTAATCTGGAACATCGTATGGGTAAGCGTAATCTGGAACATCGTATGGGTAAGCGTAATCTGGAACATCGTATGGGTAATCAACCTTCGTCGTCATGATC |
Recombinant G. lamblia cyclin B | |
3977-F (GL50803_3977) | CCCAAGCTTATGCATTAGACGACGAA |
3977-R (GL50803_3977) | CCGCTCGAGCTTTGCTTCCTTTGTATT |
Real-time PCR | |
γ-girdin-RT-F (GL50803_17230) | GCATCCGAGAGAAACATAAA |
γ-giardin-RT-R (GL50803_17230) | TTAATCAACCTTCGTCGTCA |
Actin-F (GL50803_15113) | GTCCGTCATACCATCTGTTC |
Actin-R (GL50803_15113) | GTTTCCTCCATACCACACG |
Mopholino sequences | |
Control | CCTCTTACCTCAGTTACAATTTATA |
Anti-Glγ-giardin (GL50803_17230) | CAATATAAACGCACATTGCGAAGAG |
Anti-GlMBP (GL50803_16343) | GCTGAAAACCATAGCCTCGGACATT |