Table 2 Strains and plasmids used in this study
From: Role of gamma-giardin in ventral disc formation of Giardia lamblia
Organism/plasmid | Description | Source/reference |
|---|---|---|
Giardia lamblia | ||
ATCC 30957 | Clinical isolate | ATCC |
Escherichia coli | ||
DH5α | supE44, ΔlacU169 (Φ80 lacZ ΔM15), hsdR17, recA1, endA1, gyrA96, thi-1, relA1 | Invitrogen |
BL21 (DE3) | F9, ompT, hsdSB(rB-mB-) gal, dcm (DE3) | Invitrogen |
Plasmids | ||
pGFP.pac | Shuttle vector, AmpR, pac gene | [23] |
pGlγ-giardinHAX3.pac | pGFP.pac, 936-bp encoding glγ-giardin (GiardiaDB ID GL50803_17230) | This study |
pET21b | Expression vector, AmpR | Novagen |
pET-cyclinB | pET21b, 1,026 bp encoding G. lamblia cyclin B (GiardiaDB ID GL50803_3977) | This study |