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Table 2 Strains and plasmids used in this study

From: Role of gamma-giardin in ventral disc formation of Giardia lamblia

Organism/plasmid Description Source/reference
Giardia lamblia
ATCC 30957 Clinical isolate ATCC
Escherichia coli
DH5α supE44, ΔlacU169 (Φ80 lacZ ΔM15), hsdR17, recA1, endA1, gyrA96, thi-1, relA1 Invitrogen
BL21 (DE3) F9, ompT, hsdSB(rB-mB-) gal, dcm (DE3) Invitrogen
Plasmids
pGFP.pac Shuttle vector, AmpR, pac gene [23]
pGlγ-giardinHAX3.pac pGFP.pac, 936-bp encoding glγ-giardin (GiardiaDB ID GL50803_17230) This study
pET21b Expression vector, AmpR Novagen
pET-cyclinB pET21b, 1,026 bp encoding G. lamblia cyclin B (GiardiaDB ID GL50803_3977) This study
  1. Abbreviations: Amp, ampicillin; Kan, kanamycin; R, resistant; DNA-BD, DNA binding domain; AD-activation domain; HA, hemagglutinin