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Fig. 3 | Parasites & Vectors

Fig. 3

From: Drug transporter and oxidative stress gene expression in human macrophages infected with benznidazole-sensitive and naturally benznidazole-resistant Trypanosoma cruzi parasites treated with benznidazole

Fig. 3

RT-qPCR validation of transcript expression of drug transporters and oxidative stress arrays in BZ-sensitive and BZ-resistant T cruzi-infected THP-1 MΦ cells treated with BZ. Relative mRNA expression is expressed as a ratio of transcripts in the study groups compared to transcripts in the control group (differentiated THP-1 macrophages without infection and without treatment). The values presented for the quantitative reverse-transcriptase real-time PCR analyses are the mean of the ratios of macrophage transcripts from two independent experiments. The gene expression in the study groups was normalized to GAPDH. The genes listed are MT3, metallothionein 3; AQP1, aquaporin 1; and AQP7, aquaporin 7. Significant differences were determined by two-way ANOVA, followed by Bonferroni’s multiple comparison test, *P < 0.05, **P < 0.01, and ***P < 0.001

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