Table 1 Primers for this experiment
Name | Sequence (5ʹ–3ʹ) |
|---|---|
ECR-DP | F:CTGYGACATCGACATGTACATG |
R:TCACTCYTGGATGTCCCARATCTC | |
dsECR | F:GGATCCTAATACGACTCACTATAGGCATCGTCTATGGGTGGTGGTGTGAG |
R:GGATCCTAATACGACTCACTATAGGCGATGGCTGTGAGGAGTGCATATTC | |
dsNC-1 | F:GTAGCAGGTGTGGTTCATCC |
R:CTGATGCATTGCCTTCGTCC | |
dsNC | F:GGATCCTAATACGACTCACTATAGGGTAGCAGGTGTGGTTCATCC |
R:GGATCCTAATACGACTCACTATAGGCTGATGCATTGCCTTCGTCC | |
ECR-WT-DLR | F:AGCTTTGTTTAAACGCCGAGATTTGGGACATCCAAGAG |
R:CTAGTCTAGACAGAAAAGAGGGTTAGATTCGC | |
RP-NC-DLR | F:AGCTTTGTTTAAACGCCGAGATTTGGGACATCCAAGAG |
R:CTAGTCTAGACAGAAAAGAGGGTTAGATTCGC | |
ECR-qPCR | F:GTGCCAGTGTAGGCGATTCAG |
R:CTGTATGCGCTCCACCTTGC | |
GSP-ECR-3UTR-F1 | F:TGGACCCGTGCAAGGTGGAG |
GSP-ECR-3UTR-F2 | F:ACAGAGCTGCGCACCTTGG |
β-actin | F:CGTTCCTGGGTATGGAATCG |
R:TCCACGTCGCACTTCATGAT | |
GAPDH | F:CGTGCCGCCTGGAGAAACCTG |
R:AGAGTGGGAGTTGCTGTTGAAGTCG | |
let-7-qPCR | F:ACACTCCAGCTGGTGAGGTAGTAGGT |
miR-2a-qPCR | F:ACACTCCAGCTGGTATCACAGCCAGCTT |
miR-8-qPCR | F:ACACTCCAGCTGGTAATACTGTCAGGTA |
mir-451-qPCR | F:ACACTCCAGCTGGAAACCGTTACCATTA |
miRNA-URP | R:GTCGTATCCAGTGCAGGGTCCGAGGT |
ECR-NOTI-1429 | F:CCGGAATTCATGCGACGCAAGTGCCA |
R:AAATATGCGGCCGCTGCATGAGCAGAAAAGAGGG |