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Fig. 2 | Parasites & Vectors

Fig. 2

From: Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection

Fig. 2

Immunization with Et-EmAMA1 and/or Et-EmIMP1 elicited E. maxima-specific humoral and cell-mediated immunity. a The EmAg-specific antibody production after primary and secondary immunization with recombinant Eimeria line(s) or wild-type parasites. b, c The EmAg-specific cellular immune responses after secondary immunization was analyzed by ELISPOT. The representative (b) and mean number (c) of IFN-γ-secreting lymphocytes (spots) in PBMCs from naïve (Ctrl), wild-type E. tenella (WT), Et-EmAMA1 only, Et-EmIMP1 only, and Et-EmAMA1 and Et-EmIMP1 immunized birds after stimulation with PBS, E. maxima oocyst antigens (Em Ag) and PMA plus ionomycin (PMA+Ion), (n = 3). *P < 0.05, **P < 0.01. Abbreviation: NS, no significant difference

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Parasites & Vectors

ISSN: 1756-3305

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