Table 1 List of primers used in the SYBR green-based qRT-PCR analysis
Gene | Primer sequence (5′–3′) | Gene | Primer sequence (5′–3′) |
|---|---|---|---|
GAPDH-F | ACGTGTCTGTTGTGGATCTGAC | TLR2-F | CTGGGCTGTAATCATCCTGT |
GAPDH-R | CGCTGTTGAAGTCGCAGGAG | TLR2-R | AGGTGATCTCGTTGTTGGAC |
CD1a-F | TGTGCCACGTCTCAGGATT | TLR4-F | GGTTCAAACTTCGTGGGCTT |
CD1a-R | TGTCCTGGTCTCCTAGACTGC | TLR4-R | AGCGGAGGTTTCTGAGTGAT |
MHC II-F | CCTCGCTTGCCTGAATTTGC | CD40-F | GGGCTTTTGGATACCGTCTGT |
MHC II-R | ACAGGTGCCGACTGATGC | CD40-R | AGCAGATGACACGTTGGAGAAG |
IL-12B-F | CAGGGACATCATCAAACCAG | CD80-F | CCATCCTGCCTGGAAAAGTG |
IL-12B-R | CTTGTGGCATGTGACTTTGG | CD80-R | GGTTATCGTTCATGTCAGTGATGGT |
TGF-β1-F | CGTGCTAATGGTGGAATAC | CD83-F | TGTGAAGCCCTTTGCTTGAC |
TGF-β1-R | GCCAGGAATTGTTGCTATA | CD83-R | CCAAGAGGTTGACCAGATAG |
TGF-β3-F | ACCTGGGATTAAGGACAAG | CD86-F | AGAAGGTCCCAAGGACTGGT |
TGF-β3-R | TCAAGGAAGAAGGCGAGA | CD86-R | GCTTGGCACAGGTGACTTTG |
TNF-F | TAACAAGCCGGTAGCCCACG | TNF-R | GCAAGGGCTCTTGATGGCAGA |