Fig. 3

Localization of SUB in unfed R. australis larvae with polyclonal anti-SUB rabbit serum. a A cross-section (20× magnification) of a larva stained with rabbit anti-SUB serum (1:400 diluted). The square indicates the area depicted in higher magnification (40×) in b. IgG showed binding in the acini of the salivary glands, throughout the cytoplasm and within granules (indicated with arrows). c A cross section of a larva (20× magnification) stained with naïve rabbit serum (1:400 diluted). The square indicates the area depicted in higher magnification (40×) in d. d Details of the salivary glands are depicted on the right side (40× magnification). In contrast to the polyclonal anti-SUB serum no IgG binding could be observed in the salivary glands after incubation with naïve rabbit serum. Abbreviations: Syn, synganglion; MG, midgut; RS, rectal sac; A, acinus