Novel variants of the newly emerged Anaplasma capra from Korean water deer (Hydropotes inermis argyropus) in South Korea

Table 1 PCR primers and conditions used in this study

Target gene	Primer name	Primer sequence (5′-3′)	Annealing T (°C)	Target size (bp)	Reference
rrs	Forward	TTGAGAGTTTGATCCTGGCTCAGAACG	57	1499	[12]
rrs	Reverse	WAAGGWGGTAATCCAGC	57	1499	[12]
gltA	Outer F	GCGATTTTAGAGTGYGGAGATTG	55	1031	[12]
	Outer R	TACAATACCGGAGTAAAAGTCAA	55	1031	[12]
	Inner F	TCATCTCCTGTTGCACGGTGCCC	60	594	[21]
	Inner R	CTCTGAATGAACATGCCCACCCT	60	594	[21]
groEL	Forward	GCGAGGCGTTAGACAAGTCCATT	58	1129	[12]
groEL	Reverse	TCCAGAGATGCAAGCGTGTATAG	58	1129	[12]
msp2	Outer F	GCGTGTTGATGGCTCTGGT	52	1089	[12]
	Outer R	ACCAGTATCCTTATTTTTACC	52	1089	[12]
	Inner F	GAGTGCACCAGAGCCTAGAA	56	801	This study
	Inner R	TCACCATCACCAAGCACTCT	56	801	This study
msp4	Outer F	CAGTCTGCGCCTGCTCCCTAC	55	757	[12]
	Outer R	AGGAATCTTGCTCCAAGGTTA	55	757	[12]
	Inner F	GGGTTCTGATATGGCATCTTC	56	656	[15]
	Inner R	GGGAAATGTCCTTATAGGATTCG	56	656	[15]

Abbreviations: rrs, 16S rRNA; gltA, citrate synthase; groEL, heat-shock protein; msp2, major surface protein 2; msp4, major surface protein 4; T, temperature

ISSN: 1756-3305