Fig. 3

Phylogenetic analysis of the calpains. a A rooted tree comparison of SmeCalp1 with other calpain 1 orthologs found in trematodes, cestodes, nematodes, protozoans, insects and vertebrates. b An unrooted tree comparison of calpain isoforms identified in human Schistosoma spp. The trees were constructed with maximum likelihood method using MEGA7 program, 1000 bootstrap replicates. Numbers at the nodes indicate the proportion of bootstraps. The abbreviations used and GenBank accession numbers are provided in Additional file 2: Table S2