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Fig. 4 | Parasites & Vectors

Fig. 4

From: Molecular characterization and functional analysis of the Schistosoma mekongi Ca2+-dependent cysteine protease (calpain)

Fig. 4

Expression of rSmeCalp1 and detection of native SmeCalp1 in parasite antigens. a rSmeCalp1 was successfully expressed in Escherichia coli. Lane M: PageRuler Prestained Protein Ladder (Thermo Fisher Scientific); Lane 1: non-induction; Lane 2: IPTG induction; Lane 3: soluble fraction; Lane 4: insoluble fraction; Lane 5: purified rSmeCalp1; Lane 6: refolded rSmeCalp1; Lane 7: western blot analysis of refolded rSmeCalp1 probe with anti-His tag antibody. Arrowhead indicates rSmeCalp1. b Western blot analysis detecting native SmeCalp1 in CWA of adult male and female and ES. Lane M: PageRuler Prestained Protein Ladder (Thermo Fisher Scientific Inc.); Lanes 1–4: mice number 1, 2, 3 and 4, respectively. Abbreviations: CWA, crude worm antigen; SmeES, S. mekongi excretory-secretory product

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Parasites & Vectors

ISSN: 1756-3305

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