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Table 1 Primers used in the present study to evaluate the expression of the two ABC-B transporter genes and the ABC-B half transporter gene

From: Differential ABC transporter gene expression in adult Dirofilaria immitis males and females following in vitro treatment with ivermectin, doxycycline or a combination of both

Gene

Primer name

Primer sequence (5′–3′)

Dim-pgp-10

DimmScaf48-cDNA-F8

F: GCCATCGTAGGTCCATCAGGTTCTGGT

DimmScaf48-cDNA-R12

R: TGTTCAACTGAAACGACCACACGTC

Dim-pgp-11

DimmScaf04-cDNA-F6

F: TTAACAGTGTTGATGAAGGATCAAATCC

DimmScaf04-cDNA-R6

R: ATATTTCGCTGCGGTCTTGTTGG

Dim-haf-4

DimmScaf101-cDNA-F5

F: GTGCAAACTCGAGGTTTTGCTGT

DimmScaf101-cDNA-R2

R: TCCACCTCGAAGACCTCCAGCA

18S rDNA

18SQ-F

F: GGGACAAGCGGTGTTTAGC

18SQ-R

R: GCACGCTGATTCCTCCAGT

  1. Abbreviations: F, forward; R, reverse