Assessment of a metabarcoding approach for the characterisation of vector-borne bacteria in canines from Bangkok, Thailand

Table 1 Primers used for conventional PCR, real-time PCR and taxonomic cross-validation of NGS results

Taxon targeted	PCR type	Primer pair (5′-3′)	Gene targeted	Product size (bp)	Reference
Ehrlichia canis-specific	cPCR	ECA: AACACATGCAAGTCGAACGGA	16S rRNA	400	[7]
Ehrlichia canis-specific	cPCR	HE3: TATAGGTACCGTCATTATCTTCCCTAT	16S rRNA	400	[7]
Anaplasma platys-specific	cPCR	PLATYS-F: GATTTTTGTCGTAGCTTGCTATG	16S rRNA	678	[50]
Anaplasma platys-specific	cPCR	EHR16S-R: TAGCACTCATCGTTTACAGC	16S rRNA	678	[50]
Mycoplasma spp.-specific	cPCR	HBT-F: ATACGGCCCATATTCCTACG	16S rRNA	600	[48]
Mycoplasma spp.-specific	cPCR	HBT-R: TGCTCCACCACTTGTTCA	16S rRNA	600	[48]
Rickettsia Spotted Fever Group	cPCR	ompB-F: CGACGTTAACGGTTTCTCATTCT	Outer membrane protein B (ompB)	252	[49]
Rickettsia Spotted Fever Group	cPCR	ompB-R - ACCGGTTTCTTTGTAGTTTTCGTC	Outer membrane protein B (ompB)	252	[49]
Bartonella spp.-specific	cPCR	prAPT0257: GCCTTCAAGGAGTTGATTTTGTTGTTGCCAAT	Filamenting temperature-sensitive mutant Z (ftsZ)	500	[52]
Bartonella spp.-specific	cPCR	prAPT0258: ACGACCCATTTCATGCATAACAGAAC	Filamenting temperature-sensitive mutant Z (ftsZ)	500	[52]
Filarial worm-specific	cPCR	DIDR-F1: AGTGCGAATTGCAGACGCATTGAG	5.8S-ITS2-28S	430–660	[53]
Filarial worm-specific	cPCR	DIDR-R1: AGCGGGTAATCACGACTGAGTTGA	5.8S-ITS2-28S	430–660	[53]
Rickettsia Spotted Fever and Typhus Groups	qPCR	CS-F: TCGCAAATGTTCACGGTACTTT	Citrate synthase (gltA)	74	[51]
		CS-R: TCGTGCATTTCTTTCCATTGTG
		CS-P (Probe): 6-FAM-TGCAATAGAAGAACCGTAGG CTGGATG-BHQ-1

ISSN: 1756-3305