Fig. 6

Lysosome localization of the N-terminal tagged Pmp and BspA protein. To verify the observed localizations of the N-terminal tagged Pmp and BspA protein, a co-localization with LysoTracker (red) was performed. The candidate proteins were detected using a specific α-HA antibody (green) while 4′,6-diamidin-2-phenylindol (DAPI) was used for nucleus staining (blue). In the T1 wt strain as well as the candidate proteins which carry the HA-tag at the C-terminus several small lysosomes were detected, equally distributed in the cytosol. Simultaneously the Pmp and BspA show circular structures around and near the nucleus, respectively. In contrast, one greatly enlarged lysosome could be detected in the N-terminally tagged cultures which clearly co-localizes with the HA-fusion constructs. Scale-bar: 2 µm