Fig. 1

Validation of the microarray analysis results by qPCR. Data are normalized to the internal housekeeping control PSMD4. Expression levels of 15 selected genes were determined using the comparative method (2^−ΔΔct). Data are expressed as the mean ± SEM of three independent experiments (three biological replicates). Asterisks show statistical differences (*P < 0.05, **P < 0.01) tested by one-way ANOVA with multiple comparisons (Tukey’s post-hoc test)