Fig. 2

Comparison of Chagas Sero K-SeT RDT and ELISA for TSSApep-II/V/VI serology. a Chagas Sero K-SeT RDT, which uses Protein G for IgG detection, showing coating of nitrocellulose membrane and zones for application of sample then buffer. b Results were assessed visually and accorded with ELISA using specific secondary antibodies of both humans and dogs (samples H1 and Cf1, Cf2 positive by both tests; H2 negative by both tests), and was in some cases more sensitive than ELISA (sample Cf3: positive RDT, negative ELISA). Some dog and cat sera were positive by ELISA for TSSApep-V/VI in addition to TSSApep-II/V/VI (samples Fc1 and Fc2). Chagas Sero K-SeT was not able to detect feline IgG (samples Fc1, Fc2: positive ELISA, negative RDT). ELISA results were based on absorbance unit values and the cut off was determined by comparing to T. cruzi seronegative samples