Table 3 Degree of agreement in qPCR findings for all species of soil-transmitted helminths between A and B aliquots (for individual samples) and 1 and 2 samples (for pools) from Natural History Museum (NHM) and Smith College (SC) as calculated through Cohenʼs kappa statistic
Species | Method | Field samples | Seeding | ||||
|---|---|---|---|---|---|---|---|
NHM | P-value | SC | P-value | SC | P-value | ||
N. americanus | Individual | 0.67 (0.41–0.94) | < 0.001 | – | – | 1.00 (1.00–1.00) | < 0.001 |
Pool | 0.87 (0.69–1.00) | < 0.001 | – | – | 1.00 (1.00–1.00) | < 0.001 | |
A. lumbricoides | Individual | 0.67 (0.41–0.94) | < 0.001 | 1.00 (1.00–1.00) | < 0.001 | – | – |
Pool | 0.87 (0.69–1.00) | < 0.001 | 1.00 (1.00–1.00) | < 0.001 | – | – | |
T. trichiura | Individual | 1.00 (1.00–1.00) | < 0.001 | 1.00 (1.00–1.00) | < 0.001 | – | – |
Pool | 0.66 (0.43–0.89) | < 0.001 | 0.71 (0.45–0.97) | < 0.001 | – | – | |
An. ceylanicum | Individual | 1.00 (1.00–1.00) | < 0.001 | 1.00 (1.00–1.00) | < 0.001 | – | – |
Pool | 0.70 (0.43–0.97) | < 0.001 | 1.00 (1.00–1.00) | < 0.001 | – | – | |
S. stercoralis | Individual | 0.79 (0.40–1.00) | < 0.001 | 1.00 (1.00–1.00) | < 0.001 | – | – |
Pool | 0.87 (0.62–1.00) | < 0.001 | 0.44 (− 0.01–0.91) | 0.07 | – | – | |