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Table 3 Species, physiological age grade and Cq values of the 4 specimens identified as infected with BTV by quantitative PCR for the BTV Seg10 gene

From: Potential vectors of bluetongue virus in high altitude areas of Yunnan Province, China

Specimen number

Sex

Physiological age grade

Cq value

Species identification

Based on morphology

Based on cox1 barcode

SGRL256

Female

Parous

24.01

C. obsoletus

Culicoides sp. close to C. obsoletus

SGRL298

Female

Parous

22.79

C. tainanus

C. tainanus

SGRL301

Female

Parous

21.46

C. tainanus

Culicoides sp. close to C. tainanus

SGRL354

Female

Parous

23.54

C. tainanus

C. tainanus

  1. Note: 806 Culicoides were collected, and only 4 out of 265 tested specimens had a Cq value lower than 25