|
Species
|
Target gene
|
Primer and probe
|
Sequence (5′–3′)
|
T (°C)
|
Reference
|
|---|
|
qPCR
|
|
Anaplasmataceae
|
23S rRNA
|
TtAna-F
|
TGACAGCGTACCTTTTGCAT
|
60
|
[23, 24]
|
|
TtAna-R
|
GTAACAGGTTCGGTCCTCCA
|
|
TtAna-S
|
FAM-GGATTAGACCCGAAACCAAG-TAMRA
|
|
Conventional PCR
|
|
Anaplasma spp.
|
23S rRNA
|
Ana23S-212f
|
GTTGAAAARACTGATGGTATGCA
|
55
|
[23, 24]
|
|
Ana23S-753r
|
TGCAAAAGGTACGCTGTCAC
|
|
16S rRNA
|
AENW-16S-F1
|
GCAGACGGGTGMGTAAYG
|
50
|
[73]
|
|
AENW-16S-R
|
GTGCCAGCAGCCGCGGTAAT
|
|
AENW-16S-F2 (seq.)
|
GTGCCAGCAGCCGCGGTAAT
|
|
rpoB
|
Ana-rpoBF
|
GCTGTTCCTAGGCTYTCTTACGCGA
|
55
|
[22]
|
|
Ana-rpoBR
|
AATCRAGCCAVGAGCCCCTRTAWGG
|
|
Ehrlichia spp.
|
groEL
|
Ehr-groEL-F
|
GTTGAAAARACTGATGGTATGCA
|
50
|
[22]
|
|
Ehr-groEL-R
|
ACACGRTCTTTACGYTCYTTAAC
|
|
Ticks
|
12S rDNA
|
T1B
|
AAACTAGGATTAGATACCCT
|
51
|
[21]
|
|
T2A
|
AATGAGAGCGACGGGCGATGT
|
- Abbreviation: T, annealing temperature; seq., sequencing
