Fig. 1

Ivermectin activity against P. berghei asexual and sexual blood stages in the mammalian host. a Schematics of assessment of in vivo compound activity on parasitemia, gametocytemia and exflagellation of mice treated with ivermectin. b In vivo activity of ivermectin on parasitemia of mice treated by a single oral dosage of ivermectin. Results are a representation of Syto 61 positive events on flow cytometry analysis and are expressed as the mean of parasitemia values (percentage of infected red blood cells) ± standard deviation (SD). c In vivo activity of ivermectin on female and male gametocytemia of mice treated by a single oral dosage of ivermectin. Female gametocytemia is represented by a dashed line and male gametocytemia by a solid line. Female and male gametocytes were identified by flow cytometry analysis of RFP⁺ or GFP⁺ events, respectively. Results are expressed as the mean of gametocytemia values (percentage of gametocytes) ± SD. d In vivo activity of ivermectin on the number of exflagellation events per 40× microscopic field of mice treated by a single oral dosage of selected drug. Results are expressed as the mean of observed exflagellation events ± SD. Abbreviations: ns, non-significant