Table 2 Analytical parameters of the duplex T.cruzi satDNA/IRBP qPCR assay
Analytical parameter | satDNA/IRBP qPCR |
|---|---|
Analytical sensitivity, par.eq./ml | |
TcI (Silvio X10) | 0.01 |
TcVI (CL Brener) | 0.01 |
Inclusivity (fg/µl) | |
Tc I (Silvio X10) | 0.25 |
Tc II (Y) | 0.125 |
Tc III (M5631 cl5) | 0.0625 |
Tc IV (4167) | 0.0625 |
Tc V (MnCl2) | 0.0625 |
Tc VI (CL Brener) | 0.0625 |
Exclusivity (non detectable qPCR) pg/µl | |
T. rangeli | 100 |
L. major | 1000 |
L. donovani | 1000 |
L. amazonensis | 100 |
Reportable range | |
Cl Brener | 0.1–105 par.eq/ml; y = − 2.48X + 22.69; R2 = 0.99 |
Silvio X10 | 1–104 par.eq/ml; y = − 2.64 + 27.28; R2 = 0.98 |