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Table 4 IRBP (eIAC) gene amplification in duplex satDNA/IRBP qPCR assay for T.cruzi DNA detection in samples from reservoir species

From: Development and evaluation of a duplex TaqMan qPCR assay for detection and quantification of Trypanosoma cruzi infection in domestic and sylvatic reservoir hosts

 

n

satDNA/IRBP qPCR

IRBP control

T cruzi positive/total

Valid samples

Mean Cq

SD

Wild reservoirs (n = 35)

Small rodents

 Akodon toba, Argentina

5

5

26.29

2.22

0/5

 Galea leucoblephara, Argentina

1

1

26.88

nd

0/1

 Rattus rattus, Argentina

1

1

24.72

nd

0/1

Marsupials

 Didelphis virginiana, México

4

4

24.29

1.27

0/4

 Didelphis marsupialis, Colombia

3

3

26.28

0.27

3/3

 Marmosa murina, Colombia

3

3

29.73

4.91

3/3

Bats

 Tadarida brasiliensis, Argentina

1

1

24.48

nd

0/1

 Promops nasutus, Argentina

4

4

33.60

1.97

0/4

 Desmodus rotundus (vampire), Argentina

2b

1

27.90

nd

0/1

Other mammals

 Conepatus chinga (skunk), Argentina

3

3

24.86

1.65

0/3

 Lagostomus maximus (viscacha), Argentina

3

3

28.38

0.20

0/3

 Leopardus geoffroyi (wildcat), Argentina

1

1

22.71

nd

0/1

 Lepus europaeus (hare), Argentina

1

1

27.88

nd

0/1

 Mazama gouazoubira (brown brocket deer), Argentina

1

1

26.78

nd

0/1

 Lycalopex gymnocercus (Pampas fox), Argentina

2

2

25.08

0.60

0/2

Domestic reservoirs (n =117)

 Bos taurus (cow), Argentina

12

12

24.84

0.31

0/12

 Canis lupus familiaris (dog), Argentina

27

27

24.52

1.10

0/27

 Canis lupus familiaris (dog), Méxicoa

4

4

28.85

4.50

4/4

 Capra hircus (goat), Argentina

24

24

23.75

1.13

0/24

 Equus caballus (horse), Argentina

2

2

23.40

0.49

0/2

 Felis catus (cat), Argentina

4

4

26.21

0.37

0/4

 Felis catus (cat), México

10

10

26.45

0.61

10/10

 Ovis aries (sheep), Argentina

29

29

25.16

0.92

0/29

 Ovis aries (sheep), Méxicoa

2

2

32.67

1.51

2/2

 Sus scrofa domesticus (pig), Argentina

3

3

22.72

0.98

0/3

  1. a Phenol chloroform DNA extraction
  2. b One sample was IRBP negative
  3. Note: IRBP amplification and detection of T.cruzi positive cases are shown
  4. Abbreviations: SD, standard deviation; Cq, quantification cycle; nd, not determined