Fig. 1

A pulldown assay and co-immunoprecipitation validate the interaction between TRBD and ZFD. a Binding of His-TRBD to GST-ZFD immobilized on glutathione beads was determined by WB analysis. Total protein was probed with an anti-His or anti-GST antibody as indicated. b Binding of His- C-terminal part of TERT to GST-ZFD immobilized on glutathione beads was determined by WB analysis. Total protein was probed with an anti-His or anti-GST antibody as indicated. c Binding of His-TERT to GST-ZFD immobilized on glutathione beads was determined by WB analysis. Total protein was probed with an anti-His or anti-GST antibody as indicated. d Interaction between TRBD and ZFD in 293T cells. Immunoprecipitation was detected by WB with anti-HA and anti-Myc antibodies. e TRBD-ZFD protein was detected at 10 h, 20 h, and 40 h. *P < 0.05