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Table 2 Parameters for Onchocerca volvulus peptides in the multiplex ELISA after testing on 480 plasma samples on 3 different production lots

From: Assessment of multiplex Onchocerca volvulus peptide ELISA in non-endemic tropical regions

Peptide IDSequencer2Intensity
(Q1-Median-Q3)
Spot intensity cut-off
OvMP-1VSVEPVTTQETVSV0.974.6–85.4–101.017.43
OvMP-23VSVKDGEDKVSVQTSNLDVSV0.981.5–23.7–74.512.44
OvMP-48VSVDNNGANFEVSVNLNANSNPNVSVEKDGKKVSV0.950.8–11.7–34.88.04
  1. Note: Sequences in bold represent the different epitopes. Correlation coefficients r2 are based on multiple linear regression. The signal intensity levels of the individual peptides represent 1st quartile – median – 3rd quartile. “Spot intensity cut-off” threshold of all individual peptides was calculated based on receiver operating characteristic (ROC) analysis