Fig. 5From: Diagnosing bovine parafilariosis: utility of the cytochrome c oxidase subunit 1 gene and internal transcribed spacer region for PCR detection of Parafilaria bovicola in skin biopsies and serohemorrhagic exudates of cattleDetection of P. bovicola in samples from presumably affected (Lanes 4–12) and control animals (Lanes 13–14) and a Musca sp. fly using cox1-PCR. Lane 1: marker (MassRuler® Express Forward DNA Ladder Mix, Thermo Fisher Scientific); Lane 2: no-template control; Lane 3: positive control; Lane 4: native liquid exudate (cow 1); Lane 5: native liquid exudate (cow 3); Lane 6: native liquid exudate (cow 4); Lane 7: liquid exudate fixed in 70% ethanol (cow 2); Lane 8: native liquid exudate (cow 2); Lane 9: native liquid exudate (cow 5); Lane 10: dry exudate (cow 2); Lane 11: dry exudate (cow 6); Lane 12: skin biopsy fixed in 70% ethanol (cow 7); Lane 13: EDTA blood (control cow 1); Lane 14: skin biopsy (control cow 2); Lane 15: Musca flyBack to article page