Fig. 4From: Genetic and antigenic variation of the bovine tick-borne pathogen Theileria parva in the Great Lakes region of Central AfricaMultiple amino acid sequence alignment of nine Tp2 antigen variants detected in 96 T. parva samples from DRC and Burundi. Amino acids are denoted by the single-letter codes. Var-1 to var-59 are variant names. The antigen variants nomenclature used in this study was first initiated by Pelle et al. [36]. Antigen variants var-1 and var-2 were described in Pelle et al. [36] and Salih et al. [37] and are, respectively, Muguga (identical to Serengeti-transformed) and Kiambu-5 strains. Reference sequences component of the Muguga cocktail live vaccine are represented by Muguga (GenBank: JF451856), Serengeti (Serengeti-transformed, GenBank: JF451862) and Kiambu-5 (GenBank: JF451880). The numbers in square brackets behind variants names indicate the number of T. parva samples represented by each variant. The six previously described epitopes (epitope1-6), that are the target of the bovine CD8+ T cells immune responses are bolded and boxed. The conserved amino acid residues in the epitopes are coloured in red. The star (*) below the alignment indicates positions of conserved amino acid residues. The shaded and boxed flanked regions denote the inner primers used for sequencing. Tp2 Antigen variants var-1 and var-2 are found in Muguga/Serengeti and Kiambu-5 strains, respectively. Corresponding gene alleles and sample characteristics are presented in Additional file 3: Table S3Back to article page