Genetic and antigenic variation of the bovine tick-borne pathogen Theileria parva in the Great Lakes region of Central Africa

Table 1 PCR oligonucleotide primers used for amplification of p104, Tp1 and Tp2 genes with their corresponding annealing temperatures and amplicon sizes

Gene locus	Primer name	Primer sequence (5′–3′)	Annealing temperature (°C)	Amplicon size (bp)	Reference
p104	IL3231 (Fw1)	ATTTAAGGAACCTGACGTGACTGC	60	496	[22]
	IL755 (Rev1)	TAAGATGCCGACTATTAATGACACC	60	496	[22]
	IL4243 (Fw2)	GGCCAAGGTCTCCTTCAGAATACG	55	277	[41]
	IL3232 (Rev2)	TGGGTGTGTTTCCTCGTCATCTGC	55	277	[41]
Tp1	Tp1-Fw1	ATGGCCACTTCAATTGCATTTGCC	50	432	[36]
	Tp1-Rev1	TTAAATGAAATATTTATGAGCTTC	50	432	[36]
	Tp1-Fw2	TGCATTTGCCGCTGATCCTGGATTCTG	55	405	[24, 37]
	Tp1-Rev2	TGAGCTTCGTATACACCCTCGTATTCG	55	405	[24, 37]
Tp2	Tp2-Fw1	ATGAAATTGGCCGCCAGATTA	50	525	[36]
	Tp2-Rev1	CTATGAAGTGCCGGAGGCTTC	50	525	[36]
	Tp2-Fw2	ATTAGCCTTTACTTTATTATTTWCATTYTAC	54	504	[24, 37]
	Tp2-Rev2	CTATGAAGTGCCGGAGGCTTCTCCT	54	504	[24, 37]

Abbreviations: Fw1, forward outer; Fw2, forward inner; Rev1, reverse outer; Rev2, reverse inner

ISSN: 1756-3305