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Fig. 9 | Parasites & Vectors

Fig. 9

From: Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR

Fig. 9

Average Cq-values of quantitative PCR for Plate (vii). Cryptosporidium parvum oocysts inoculated onto HCT-8 monolayer plate C (5 × 104–50) and 10-fold dilutions of 5 × 104 (×10, ×100 and ×1000). Error bars represent the standard deviation of the mean (SD) from 2 biological repeats for cell culture data and SD from two technical repeats of each dilution for the template dilution data. Asterisks indicate significantly increased Cq signal (P < 0.05) compared to cell culture derived values

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