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Table 5 Cq values of quantitative PCR Plate (vi)

From: Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR

Oocyst quantity

TR1

TR2

1,000,000

27.70

27.36

Mean TR ± SD

27.53 ± 0.17

 

100,000

30.12

29.32

Mean TR ± SD

29.72 ± 0.40

 

10,000

32.75

32.34

Mean TR ± SD

32.55 ± 0.21

 

1000

36.70

35.78

Mean TR ± SD

36.24 ± 0.46

 

100

41.17

40.72

Mean TR ± SD

40.95 ± 0.23

 

MQ

na

na

Mean TR ± SD

na

 
  1. Notes: Pure oocyst serial dilution B. Baseline threshold 19.88 RFU
  2. Abbreviations: TR, technical repeat; SD, standard deviation; na, no DNA amplification