Skip to main content

Table 5 Cq values of quantitative PCR Plate (vi)

From: Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR

Oocyst quantityTR1TR2
1,000,00027.7027.36
Mean TR ± SD27.53 ± 0.17 
100,00030.1229.32
Mean TR ± SD29.72 ± 0.40 
10,00032.7532.34
Mean TR ± SD32.55 ± 0.21 
100036.7035.78
Mean TR ± SD36.24 ± 0.46 
10041.1740.72
Mean TR ± SD40.95 ± 0.23 
MQnana
Mean TR ± SDna 
  1. Notes: Pure oocyst serial dilution B. Baseline threshold 19.88 RFU
  2. Abbreviations: TR, technical repeat; SD, standard deviation; na, no DNA amplification