Fig. 4

The influence of PGRP-LA on PM synthesis. a Relative expression levels of PM-related genes in dsRNA-treated mosquitoes 24 h, 36 h and 48 h post-infectious blood meal. Error bars indicate standard error of the mean (n = 8). Results from one of three independent experiments are shown. Significance was determined by Student’s t-test (for details, see Additional file 3: Text S1). *P < 0.05. b Western blot of Per1 in dsGFP-treated and dsLA-treated mosquitoes. Actin was used as a loading control. Results from one of three independent experiments are shown. c PAS staining of PM in dsGFP-treated and dsLA-treated mosquitoes at 100× and 200× magnification. Red arrowheads denote the PM. Results from one of three independent experiments are shown. Scale-bars: c, 100 μm