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Table 2 Degree of concordance between cytological karyotype and individual PCR-RFLP genotyping assay

From: Highly specific PCR-RFLP assays for karyotyping the widespread 2Rb inversion in malaria vectors of the Anopheles gambiae complex

Country

DraIII concordance (%)

MspAI concordance (%)

TatI concordance (%)

An. coluzzii

An. gambiae

An. coluzzii

An. gambiae

An. coluzzii

An. gambiae

Benin

19/19 (100)

na

19/19 (100)

na

15/19 (78.9)

na

Burkina Faso

na

19/20 (95.0)

na

14/20 (70)

na

17/20 (85)

Burkina Faso (lab)

25/25 (100)

na

26/27 (96.3)

na

16/24 (66.6)

na

Cameroon

10/10 (100)

263/276 (95.2)

10/10 (100)

208/274 (75.9)

10/10 (100)

253/275 (92)

Gambia + Senegala

6/6 (100)

56/58 (96.6)

5/6 (83.3)

52/56 (92.9)

4/6 (66.7)

47/47 (100)

Guinea Bissau

3/3 (100)

18/18 (100)

3/3 (100)

16/16 (100)

3/3 (100)

16/16 (100)

Mali

201/211 (95.3)

55/57 (96.5)

128/131 (97.7)

22/26 (84.6)

104/132 (78.8)

21/28 (75)

Tanzania + DRCa

na

11/13 (84.6)

na

13/13 (100)

na

13/13 (100)

Total

264/274 (96.4)

422/443 (95.3)

191/196 (97.4)

325/405 (80.2)

152/194 (78.3)

367/399 (92.0)

  1. aSpecimens were combined into one sample due to geographical proximity and small sample size
  2. Abbreviations: DRC, Democratic Republic of Congo; na, not available